Wollner D A, Catterall W A
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8424-8. doi: 10.1073/pnas.83.21.8424.
Affinity-purified antibodies against the sodium channel from rat brain were employed to localize sodium channels in the retina by immunocytochemical procedures. In rat retina, intense staining was observed in the ganglion cell axon layer and light staining was detected in fibers of the inner plexiform layer. In frog retina, only the ganglion cell axon layer was stained. Examination at higher magnification revealed that axon hillocks and initial segments of ganglion cells had a high density of immunoreactive sodium channels, whereas the cell bodies were devoid of stain. The sharply defined region of high sodium channel density at the axon hillock is likely to be responsible for the low threshold for action potential initiation in this region of vertebrate central neurons.
利用针对大鼠脑钠通道的亲和纯化抗体，通过免疫细胞化学方法在视网膜中定位钠通道。在大鼠视网膜中，在神经节细胞轴突层观察到强烈染色，在内网状层纤维中检测到轻度染色。在青蛙视网膜中，只有神经节细胞轴突层被染色。更高倍率观察显示，神经节细胞的轴丘和起始节段具有高密度的免疫反应性钠通道，而细胞体没有染色。轴丘处钠通道密度高且界限清晰的区域可能是脊椎动物中枢神经元该区域动作电位起始阈值低的原因。
