Bitgul Gulsah, Tekmen Isil, Keles Didem, Oktay Gulgun
Department of Histology and Embryology, Medical Faculty of Dokuz Eylül University, Balcova, 35340 İzmir, Turkey.
ISRN Urol. 2013 Nov 6;2013:278720. doi: 10.1155/2013/278720. eCollection 2013.
Objective. The aim of this study was to investigate protective effects of resveratrol, a strong antioxidant, against possible negative effects of chronic immobilization stress on testes of male rats histochemically, immunohistochemically, ultrastructurally, and biochemically. Material and Methods. Male Wistar rats were divided into 4 groups (n = 7). Group I, control group (C), was not exposed to stress. Group II, stress group (S), was exposed to chronic immobilization stress. In Group III, low dose resveratrol + stress group (LRS), rats were given 10 mg/kg/day resveratrol just before the stress application. In Group IV, high dose resveratrol + stress group (HRS), rats were given 20 mg/kg/day resveratrol just before the stress application. For chronic immobilization stress application animals were put in the plastic tubes (6 cm in diameter, 15 cm in length) during 32 days for 6 hours. All animals were sacrificed 18 hours after the last stress application. Results. Histochemical and ultrastructural investigations showed that in stress group there was germ cell deprivation in seminiferous tubules and increase of connective tissue on interstitial area. No significant changes were seen in low and high dose resveratrol groups. After immunohistochemical investigations, TUNEL (+) and Active Caspase-3 (+) cells were increased in seminiferous tubules of stress group compared with those control group, but they were decreased in low and high dose resveratrol groups. According to biochemically results, MDA, GSH, and testosterone levels in stress group showed no significant difference when compared with those of the other groups. Conclusion. The chronic immobilization stress increases oxidative stress and apoptosis and causes histological tissue damages; resveratrol can minimize the histological damage in testes significantly.
目的。本研究旨在从组织化学、免疫组织化学、超微结构和生物化学方面，研究强抗氧化剂白藜芦醇对慢性制动应激可能对雄性大鼠睾丸产生的负面影响的保护作用。材料与方法。雄性Wistar大鼠分为4组（n = 7）。第一组为对照组（C），未遭受应激。第二组为应激组（S），遭受慢性制动应激。第三组为低剂量白藜芦醇+应激组（LRS），大鼠在施加应激前给予10毫克/千克/天的白藜芦醇。第四组为高剂量白藜芦醇+应激组（HRS），大鼠在施加应激前给予20毫克/千克/天的白藜芦醇。对于慢性制动应激施加，将动物置于塑料管（直径6厘米，长度15厘米）中32天，每天6小时。在最后一次应激施加后18小时处死所有动物。结果。组织化学和超微结构研究表明，应激组生精小管中有生殖细胞缺失，间质区域结缔组织增加。低剂量和高剂量白藜芦醇组未见明显变化。免疫组织化学研究后，与对照组相比，应激组生精小管中TUNEL（+）和活化半胱天冬酶-3（+）细胞增加，但在低剂量和高剂量白藜芦醇组中减少。根据生化结果，应激组的丙二醛、谷胱甘肽和睾酮水平与其他组相比无显著差异。结论。慢性制动应激会增加氧化应激和细胞凋亡，并导致组织学损伤；白藜芦醇可显著减轻睾丸的组织学损伤。
