Ganjewala Deepak, Gupta Ashish Kumar
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector-125, Noida-201303 (UP), India.
Adv Pharm Bull. 2013;3(2):379-84. doi: 10.5681/apb.2013.061. Epub 2013 Aug 20.
To evaluate phytochemical composition, antibacterial and antioxidant properties of methanolic extracts of different parts viz., leaves, follicles and latex of Indian devil tree (Alstonia scholaris Linn.) R. Br.
Antibacterial activities of the methanol extracts against Gram +ve (Bacillus subtilis and Staphylococcus aureus) and Gram -ve (Escherichia coli, Pseudomonas aeruginosa) bacteria were determined by well diffusion techniques. Aantioxidant profiles of methanol extracts were determined by 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging, superoxide anion radial scavenging and ferric thiocyanate reducing assays.
Phytochemical composition revealed abundance of flavonoids (97.3 mg QE/g DW), proanthocynidins (99.3 mg CE/g DW) and phenolics (49.7 mgGAE/g DW) in the leaf extract. Extracts of follicles and latex had comparatively very content of phenolics, flavonoids and proanthocyanidins. However, in follicle extract level of proanthocyanidins was significantly higher (46.8 mg CE/gDW). Latex extract among others exhibited most potent antibacterial activity. All the extracts displayed strong DPPH free radical and superoxide anion scavenging activities, only leaf extract displayed powerful reducing and ferrous ion chelating activities.
Study revealed significant antioxidant activities of A. scholaris leaf, follicles and latex extracts and potential antibacterial activity of latex extract.
评估印度鸡骨常山（Alstonia scholaris Linn.）R. Br.不同部位（即叶子、蓇葖果和乳汁）的甲醇提取物的植物化学成分、抗菌和抗氧化特性。
采用打孔扩散法测定甲醇提取物对革兰氏阳性菌（枯草芽孢杆菌和金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌、铜绿假单胞菌）的抗菌活性。通过1,1-二苯基-2-苦基肼（DPPH）自由基清除、超氧阴离子自由基清除和硫氰酸铁还原试验测定甲醇提取物的抗氧化特性。
植物化学成分分析表明，叶提取物中富含黄酮类化合物（97.3 mg QE/g DW）、原花青素（99.3 mg CE/g DW）和酚类化合物（49.7 mg GAE/g DW）。蓇葖果和乳汁提取物中的酚类、黄酮类和原花青素含量相对较低。然而，蓇葖果提取物中原花青素的含量显著较高（46.8 mg CE/g DW）。乳汁提取物表现出最强的抗菌活性。所有提取物均表现出较强的DPPH自由基和超氧阴离子清除活性，只有叶提取物表现出较强的还原和亚铁离子螯合活性。
研究表明，印度鸡骨常山的叶、蓇葖果和乳汁提取物具有显著的抗氧化活性，乳汁提取物具有潜在的抗菌活性。
