Zare Shahneh Fateme, Baradaran Behzad, Orangi Mona, Zamani Fatemeh
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2013;3(2):453-5. doi: 10.5681/apb.2013.074. Epub 2013 Aug 20.
The aim of this study was to investigate in vitro cytotoxic activity of four methanolic crude plant extracts against panel cell lines.
Methanolic extracts were tested for their possible antitumor activity and cytotoxicity using the 3-(4,5-dimetylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay on six cancer cell lines; non-Hodgkin's B-cell lymphoma (Raji), human leukemic monocyte lymphoma (U937), human acute myelocytic leukemia (KG-1A), human breast carcinoma (MCF-7 cells), human Prostate Cancer (PC3) and mouse fibrosarcoma (WEHI-164) cell lines and one normal cell line; Human Umbilical Vein Endothelial Cells (HUVEC).
All species showed dose dependent inhibition of cell proliferation. IC50 values ranging from 25.66±1.2 to 205.11±1.3 μg/ml. The highest cytotoxic activity Chelidonium majus L> Ferulago Angulata DC> Echinophora platyloba DC> Salvia officinalis L, respectively.
all extracts demonstrate promising cytotoxicity activity as a natural resource for future bio-guided fractionation and isolation of potential antitumor agents.
本研究旨在调查四种植物甲醇粗提物对一组细胞系的体外细胞毒性活性。
使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法，对六种癌细胞系；非霍奇金B细胞淋巴瘤（Raji）、人白血病单核细胞淋巴瘤（U937）、人急性髓细胞白血病（KG-1A）、人乳腺癌（MCF-7细胞）、人前列腺癌（PC3）和小鼠纤维肉瘤（WEHI-164）细胞系以及一种正常细胞系；人脐静脉内皮细胞（HUVEC），测试甲醇提取物的可能抗肿瘤活性和细胞毒性。
所有物种均显示出剂量依赖性的细胞增殖抑制。IC50值范围为25.66±1.2至205.11±1.3μg/ml。细胞毒性活性最高的分别是白屈菜> Ferulago Angulata DC> 扁叶刺芹DC> 鼠尾草。
所有提取物均表现出有前景的细胞毒性活性，可作为未来生物导向分级分离和潜在抗肿瘤药物分离的天然资源。
