Neophytou Andreas M, Hart Jaime E, Cavallari Jennifer M, Smith Thomas J, Dockery Douglas W, Coull Brent A, Garshick Eric, Laden Francine
Exposure Epidemiology and Risk Program, Department of Environmental Health, Harvard School of Public Heath, Boston, MA, USA.
Environ Health. 2013 Dec 7;12:105. doi: 10.1186/1476-069X-12-105.
Experimental evidence suggests that inhaled particles from vehicle exhaust have systemic effects on inflammation, endothelial activation and oxidative stress. In the present study we assess the relationships of short-term exposures with inflammatory endothelial activation and oxidative stress biomarker levels in a population of trucking industry workers.
Blood and urine samples were collected pre and post-shift, at the beginning and end of a workweek from 67 male non-smoking US trucking industry workers. Concurrent measurements of microenvironment concentrations of elemental and organic carbon (EC & OC), and fine particulate matter (PM2.5) combined with time activity patterns allowed for calculation of individual exposures. Associations between daily and first and last-day average levels of exposures and repeated measures of intercellular and vascular cell adhesion molecule-1 (ICAM-1 & VCAM-1), interleukin 6 (IL-6) and C-reactive protein (CRP) blood levels and urinary 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) were assessed using linear mixed effects models for repeated measures.
There was a statistically significant association between first and last-day average PM2.5 and 8-OHdG (21% increase, 95% CI: 2, 42%) and first and last-day average OC and IL-6 levels (18% increase 95% CI: 1, 37%) per IQR in exposure. There were no significant findings associated with EC or associations suggesting acute cross-shift effects.
Our findings suggest associations between weekly average exposures of PM2.5 on markers of oxidative stress and OC on IL-6 levels.
实验证据表明，车辆尾气中的吸入颗粒物对炎症、内皮细胞活化和氧化应激具有全身效应。在本研究中，我们评估了短期暴露与卡车运输行业工人人群中炎症性内皮细胞活化及氧化应激生物标志物水平之间的关系。
从67名美国男性非吸烟卡车运输行业工人中，在轮班前和轮班后、工作周开始和结束时采集血液和尿液样本。同时测量元素碳和有机碳（EC和OC）以及细颗粒物（PM2.5）的微环境浓度，并结合时间活动模式来计算个体暴露量。使用重复测量的线性混合效应模型评估每日、第一天和最后一天的平均暴露水平与细胞间黏附分子-1和血管细胞黏附分子-1（ICAM-1和VCAM-1）、白细胞介素6（IL-6）和C反应蛋白（CRP）血液水平以及尿8-羟基-2'-脱氧鸟苷（8-OHdG）重复测量值之间的关联。
第一天和最后一天的平均PM2.5与8-OHdG之间存在统计学显著关联（增加21%，95%置信区间：2%，42%），暴露量每增加一个四分位间距，第一天和最后一天的平均OC与IL-6水平之间也存在统计学显著关联（增加18%，95%置信区间：1%，37%）。未发现与EC相关的显著结果，也没有表明急性轮班效应的关联。
我们的研究结果表明，每周平均暴露的PM2.5与氧化应激标志物之间以及OC与IL-6水平之间存在关联。
