Ocean College, Zhejiang University, Hangzhou 310058, China.
Mar Drugs. 2013 Dec 2;11(12):4761-72. doi: 10.3390/md11124761.
Two novel cyclodepsipeptides containing an unusual anthranilic acid dimer and a D-phenyllactic acid residues, clavatustides A and B, were identified from cultured mycelia and broth of Aspergillus clavatus C2WU isolated from Xenograpsus testudinatus, which lives at extreme, toxic habitat around the sulphur-rich hydrothermal vents in Taiwan Kueishantao. This is the first example of cyclopeptides containing an anthranilic acid dimer in natural products, and the first report of microbial secondary metabolites from the hydrothermal vent crab. Clavatustides A and B suppressed the proliferation of hepatocellular carcinoma (HCC) cell lines (HepG2, SMMC-7721 and Bel-7402) in a dose-dependent manner, and induced an accumulation of HepG2 cells in G1 phase and reduction of cells in S phase.
两种新型环二肽，含有一个不寻常的邻氨基苯甲酸二聚体和一个 D-苯乳酸残基，分别命名为clavastustides A 和 B，从生活在台湾龟山岛富含硫的热液喷口极端有毒生境中的 Xenograpsus testudinatus 中分离的 Aspergillus clavatus C2WU 的培养菌丝体和发酵液中鉴定得到。这是天然产物中环二肽含有邻氨基苯甲酸二聚体的首例，也是热液喷口蟹微生物次生代谢产物的首次报道。Clavatustides A 和 B 以剂量依赖的方式抑制肝癌（HCC）细胞系（HepG2、SMMC-7721 和 Bel-7402）的增殖，并诱导 HepG2 细胞在 G1 期积累和 S 期细胞减少。
