用磺化γ球蛋白处理的组织切片中肾小球内沉积免疫复合物的修饰。
Modification of immune complexes deposited in glomeruli in tissue sections treated with sulfonized gamma-globulin.
作者信息
Sato M, Kojima H, Koshikawa S
出版信息
Clin Exp Immunol. 1986 Jun;64(3):623-8.
The modification of immune complexes (IC) deposited in renal tissues obtained from the patients with membranous nephropathy (MN), membranoproliferative glomerulonephritis (MGPN), systemic lupus erythematosus (SLE), IgA nephropathy, and rabbits with chronic serum sickness was investigated by an immunofluorescent technique. Following treatment with sulfonized gamma-globulin (S-GG), IgG deposited in glomeruli disappeared. However, the deposition of other immunoglobulins, complement and protein A, and the fixation of guinea pig complement were not influenced in spite of the disappearance of IgG deposits. In addition, deposition of antigen was demonstrated following treatment with S-GG in experimental animals. By elution with 0.02 M citrate buffer at pH 3.2, the intensity of these deposits markedly decreased or disappeared completely. The data suggests that the phenomena of IgG-dispersion from IC following the treatment with either S-GG or acid buffer are essentially different. The exact mechanisms of selective IgG-dispersion by the treatment with S-GG are unclear, but an antiglobulin activity (rheumatoid factor or anti-idiotypic antibody) of deposited IC may play an important role.
采用免疫荧光技术研究了膜性肾病（MN）、膜增生性肾小球肾炎（MGPN）、系统性红斑狼疮（SLE）、IgA肾病患者及慢性血清病兔肾组织中沉积的免疫复合物（IC）的变化。用磺化γ球蛋白（S-GG）处理后，肾小球中沉积的IgG消失。然而，尽管IgG沉积物消失，但其他免疫球蛋白、补体和蛋白A的沉积以及豚鼠补体的固定并未受到影响。此外，在实验动物中用S-GG处理后证实有抗原沉积。用pH 3.2的0.02 M柠檬酸盐缓冲液洗脱后，这些沉积物的强度明显降低或完全消失。数据表明，用S-GG或酸性缓冲液处理后IC中IgG分散的现象本质上是不同的。用S-GG处理选择性IgG分散的确切机制尚不清楚，但沉积的IC的抗球蛋白活性（类风湿因子或抗独特型抗体）可能起重要作用。
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