BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Dep. IFA-Tulln, Division Environmental Biotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria.
BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Dep. IFA-Tulln, Division Environmental Biotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria.
Vet Microbiol. 2014 Jan 10;168(1):240-4. doi: 10.1016/j.vetmic.2013.11.014. Epub 2013 Nov 22.
Acquired antibiotic resistances have been reported in lactobacilli of various animal and food sources, but there are no data from wild boar. The objective was a preliminary examination of the antibiotic resistance prevalence of intrinsically vancomycin-resistant lactobacilli isolated from wild boar intestines and analysis of the genetic determinants implicated. Out of three wild boars, 121 lactobacilli were recovered and grouped according to their whole cell protein patterns. Initial phenotypic screening revealed that all were susceptible to erythromycin (2 μg/ml), but 30 were resistant to tetracycline (32 μg/ml). Based on Randomly Amplified Polymorphic DNA-PCR clustering, 64 strains were selected as representative genotypes for identification and minimum inhibitory concentration (MIC) determination. Partial 16S rRNA gene sequencing identified four species: (i) L. mucosae (n=57), (ii) L. reuteri (n=47), (iii) L. fermentum (n=12), and (iv) L. murinus (n=5). Most heterofermentative strains displayed low MICs for ampicillin (AMP), chloramphenicol (CHL), streptomycin (STR), kanamycin (KAN), gentamicin (GEN), erythromycin (ERY), quinupristin/dalfopristin (Q/D), and clindamycin (CLI). Atypical MICs were found mainly in L. mucosae and L. reuteri for TET, KAN, STR, AMP and CHL, but except the TET MICs of L. mucosae mostly at low level. L. murinus strains revealed atypical MICs for aminoglycosides, and/or CHL, AMP, CLI. PCR screening detected tet(W) in 12 and tet(M) in one of heterofermentative strains, as well as the aph(3')-III kanamycin gene in L. murinus. This is the first report showing acquired antibiotic resistance determinants in intestinal lactobacilli of wild boar origin.
从各种动物和食物来源的乳杆菌中已经报道了获得性抗生素耐药性，但野猪中没有数据。本研究的目的是初步检测从野猪肠道中分离的固有万古霉素耐药乳杆菌的抗生素耐药流行情况，并分析涉及的遗传决定因素。从三头野猪中回收了 121 株乳杆菌，并根据其全细胞蛋白图谱进行分组。初始表型筛选显示，所有乳杆菌对红霉素（2 μg/ml）均敏感，但 30 株对四环素（32 μg/ml）耐药。基于随机扩增多态性 DNA-PCR 聚类，选择 64 株作为代表性基因型进行鉴定和最小抑菌浓度（MIC）测定。部分 16S rRNA 基因测序鉴定出 4 个种：（i）L. mucosae（n=57），（ii）L. reuteri（n=47），（iii）L. fermentum（n=12）和（iv）L. murinus（n=5）。大多数异型发酵乳杆菌对氨苄西林（AMP）、氯霉素（CHL）、链霉素（STR）、卡那霉素（KAN）、庆大霉素（GEN）、红霉素（ERY）、奎奴普丁/达福普汀（Q/D）和克林霉素（CLI）的 MIC 值较低。L. mucosae 和 L. reuteri 中的 TET、KAN、STR、AMP 和 CHL 的 MIC 值主要为非典型值，但除 L. mucosae 的 TET MIC 值外，大多数为低水平。L. murinus 菌株对氨基糖苷类药物和/或 CHL、AMP、CLI 的 MIC 值为非典型值。PCR 筛选检测到 12 株异型发酵乳杆菌中的 tet(W)和 1 株中的 tet(M)，以及 L. murinus 中的 aph(3')-III 卡那霉素基因。这是首次报道野猪肠道乳杆菌中存在获得性抗生素耐药决定因素。
