Department of Medicinal Sciences, University Hospital, Uppsala University , Sweden.
Scand J Clin Lab Invest. 2014 Mar;74(2):143-8. doi: 10.3109/00365513.2013.864785. Epub 2013 Dec 11.
While the potential toxicity of metals in humans is a well-established field of research, there are few studies that examine circulating concentrations of metals in large population-based samples. The aim of this study was to analyze levels of heavy metals and trace elements in both whole blood and serum in an elderly population, and to examine if gender, kidney function, haemoglobin or serum albumin could impact the distribution of metals between whole blood and serum.
Whole blood and serum samples from 1016 70-year-olds living in Uppsala, Sweden, were analyzed for aluminium, cadmium, cobalt, copper, chromium, mercury, manganese, molybdenum, nickel, lead, and zinc using inductively coupled plasma-sector field mass spectrometry (ICP-SFMS). Distribution between whole blood and serum was evaluated by the ratio between whole blood and serum concentration (B/S-ratio).
Concentrations differed significantly between whole blood and serum measurements for all 11 metals (p < 0.00001). The highest B/S-ratios were found for lead (27), zinc (9), manganese (6), and nickel (4). Copper (0.86), cobalt (0.84), and molybdenum (0.86) showed B/S-ratios < 1. Especially the B/S-ratios for chromium, mercury and nickel correlated with kidney function (GFR) (r = 0.21, - 0.21 and - 0.36 respectively, p < 0.0001).
The distribution between whole blood and serum varied considerably for different metals. This distribution correlated with physiological factors, mainly with kidney function, for several of the metals.
虽然金属在人体内的潜在毒性是一个成熟的研究领域，但很少有研究检查大型人群样本中金属的循环浓度。本研究旨在分析老年人全血和血清中重金属和微量元素的水平，并研究性别、肾功能、血红蛋白或血清白蛋白是否会影响金属在全血和血清之间的分布。
使用电感耦合等离子体质谱法（ICP-SFMS）分析了来自瑞典乌普萨拉 1016 名 70 岁老年人的全血和血清样本中的铝、镉、钴、铜、铬、汞、锰、钼、镍、铅和锌。通过全血与血清浓度之比（B/S 比值）评估全血与血清之间的分布。
所有 11 种金属的全血和血清测量值之间的浓度差异均具有统计学意义（p<0.00001）。铅（27）、锌（9）、锰（6）和镍（4）的 B/S 比值最高。铜（0.86）、钴（0.84）和钼（0.86）的 B/S 比值<1。特别是铬、汞和镍的 B/S 比值与肾功能（GFR）相关（r=0.21、-0.21 和-0.36，分别为 p<0.0001）。
不同金属之间全血和血清之间的分布差异很大。这种分布与生理因素有关，对于几种金属，主要与肾功能有关。
