Hochman Bernardo, Pinfildi Carlos E, Nishioka Michele A, Furtado Fabianne, Bonatti Silvilena, Monteiro Paola K P, Antunes Arainy S, Quieregatto Paulo R, Liebano Richard E, Chadi Gerson, Ferreira Lydia Masako
Universidade Federal de São Paulo (UNIFESP), Rua Napoleão de Barros, 715, 4° andar, Vila Clementino, 04023-002, São Paulo, São Paulo, Brazil,
Lasers Med Sci. 2014 May;29(3):1203-8. doi: 10.1007/s10103-013-1494-z. Epub 2013 Dec 15.
The phototherapy effects in the skin are related to biomodulation, usually to accelerate wound healing. However, there is no direct proof of the interrelation between the effects of low-level laser therapy (LLLT) and light-emitting diode (LED) in neuropeptide secretion, these substances being prematurely involved in the neurogenic inflammation phase of wound healing. This study therefore focused on investigating LLLT and LED in Calcitonin gene-related peptide (CGRP) and substance P (SP) secretion in healthy rat skin. Forty rats were randomly distributed into five groups with eight rats each: Control Group, Blue LED Group (470 nm, 350 mW power), Red LED Group (660 nm, 350 mW power), Red Laser Group (660 nm, 100 mW power), and Infrared Laser Group (808 nm, 100 mW power) (DMC® Equipamentos Ltda., São Carlos, São Paulo, Brazil). The skin of the animals in the experimental groups was irradiated using the punctual contact technique, with a total energy of 40 J, single dose, standardized at one point in the dorsal region. After 14 min of irradiation, the skin samples were collected for CGRP and SP quantification using western blot analysis. SP was released in Infrared Laser Group (p = 0.01); there was no difference in the CGRP secretion among groups. Infrared (808 nm) LLLT enhances neuropeptide SP secretion in healthy rat skin.
皮肤中的光疗效果与生物调节有关，通常是为了加速伤口愈合。然而，目前尚无直接证据表明低强度激光疗法（LLLT）和发光二极管（LED）在神经肽分泌方面的作用之间存在相互关系，而这些物质过早地参与了伤口愈合的神经源性炎症阶段。因此，本研究着重调查LLLT和LED对健康大鼠皮肤中降钙素基因相关肽（CGRP）和P物质（SP）分泌的影响。40只大鼠被随机分为五组，每组8只：对照组、蓝色LED组（470 nm，350 mW功率）、红色LED组（660 nm，350 mW功率）、红色激光组（660 nm，100 mW功率）和红外激光组（808 nm，100 mW功率）（DMC® Equipamentos Ltda.，巴西圣保罗州圣卡洛斯市）。使用点状接触技术对实验组动物的皮肤进行照射，总能量为40 J，单剂量，在背部区域的一个点进行标准化照射。照射14分钟后，收集皮肤样本，采用蛋白质印迹分析法定量检测CGRP和SP。红外激光组释放了SP（p = 0.01）；各组之间CGRP分泌无差异。红外（808 nm）LLLT可增强健康大鼠皮肤中神经肽SP的分泌。
