Smith Donna C, Wiecinski Paige N, Coggins Christopher R E, Banty Tamara H, Oldham Michael J
Regulatory Affairs, Altria Client Services, Richmond, VA, USA .
Inhal Toxicol. 2013;25 Suppl 2:34-45. doi: 10.3109/08958378.2013.854432.
Cigarettes often have a small identifying mark (monogram) printed either on the cigarette paper toward the filter end of the cigarette or on the tipping paper.
A battery of tests was used to compare the toxicology of mainstream smoke from experimental cigarettes manufactured with different monogram inks. Cigarettes with different concentrations of different pigments were compared with cigarettes without ink, and with a control ink.
Smoke from each of the experimental cigarettes was evaluated using analytical chemistry and in vitro bacterial mutagenicity (Salmonella, five strains, ± S9) and cytotoxicity (neutral red uptake) assays.
No differences were observed between experimental cigarettes printed with three different pigment loads of iron oxide-based Black pigment and non-printed cigarettes. In general, no dose response was observed. However, increases in certain smoke constituents were found to correlate with Pigment Yellow 14 (also known as benzidine yellow) and Pigment Blue 15 (copper phthalocyanine). Increases in bacterial mutagenicity were observed for high-level print of Pigment Yellow 14 in TA98 and TA1537 and the high-level print of Pigment Blue 15 in TA98. In vitro cytotoxicity of mainstream smoke was unaffected by the presence of monogram ink on cigarettes.
Statistically significant dose-responsive constituent changes and an increase in mutagenicity were observed with inclusion of Pigment Yellow 14 and Pigment Blue 15. Other pigments showed minimal toxicological activity.
香烟通常在靠近过滤嘴一端的卷烟纸或水松纸上印有一个小的识别标记（字母组合图案）。
使用一系列测试来比较用不同字母组合图案油墨制造的实验香烟主流烟气的毒理学。将含有不同浓度不同颜料的香烟与无油墨香烟以及一种对照油墨香烟进行比较。
使用分析化学以及体外细菌诱变性（沙门氏菌，五种菌株，±S9）和细胞毒性（中性红摄取）试验对每支实验香烟的烟气进行评估。
印有三种不同颜料含量的氧化铁基黑色颜料的实验香烟与未印刷香烟之间未观察到差异。一般来说，未观察到剂量反应。然而，发现某些烟气成分的增加与颜料黄14（也称为联苯胺黄）和颜料蓝15（铜酞菁）相关。在TA98和TA1537中，颜料黄14的高含量印刷以及在TA98中颜料蓝15的高含量印刷观察到细菌诱变性增加。香烟上字母组合图案油墨的存在对主流烟气的体外细胞毒性没有影响。
加入颜料黄14和颜料蓝15后，观察到具有统计学意义的剂量反应成分变化和诱变性增加。其他颜料显示出最小的毒理学活性。
