Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America ; Lehrstuhl für BioMolekulare Optik, Ludwig-Maximilians-Universität München, Munich, Germany.
PLoS One. 2013 Dec 11;8(12):e81499. doi: 10.1371/journal.pone.0081499. eCollection 2013.
We demonstrate in vivo choriocapillaris and choroidal microvasculature imaging in normal human subjects using optical coherence tomography (OCT). An ultrahigh speed swept source OCT prototype at 1060 nm wavelengths with a 400 kHz A-scan rate is developed for three-dimensional ultrahigh speed imaging of the posterior eye. OCT angiography is used to image three-dimensional vascular structure without the need for exogenous fluorophores by detecting erythrocyte motion contrast between OCT intensity cross-sectional images acquired rapidly and repeatedly from the same location on the retina. En face OCT angiograms of the choriocapillaris and choroidal vasculature are visualized by acquiring cross-sectional OCT angiograms volumetrically via raster scanning and segmenting the three-dimensional angiographic data at multiple depths below the retinal pigment epithelium (RPE). Fine microvasculature of the choriocapillaris, as well as tightly packed networks of feeding arterioles and draining venules, can be visualized at different en face depths. Panoramic ultra-wide field stitched OCT angiograms of the choriocapillaris spanning ∼32 mm on the retina show distinct vascular structures at different fundus locations. Isolated smaller fields at the central fovea and ∼6 mm nasal to the fovea at the depths of the choriocapillaris and Sattler's layer show vasculature structures consistent with established architectural morphology from histological and electron micrograph corrosion casting studies. Choriocapillaris imaging was performed in eight healthy volunteers with OCT angiograms successfully acquired from all subjects. These results demonstrate the feasibility of ultrahigh speed OCT for in vivo dye-free choriocapillaris and choroidal vasculature imaging, in addition to conventional structural imaging.
我们使用光学相干断层扫描（OCT）在正常人体中演示脉络膜毛细血管和脉络膜微血管成像。开发了一种超高速扫频源 OCT 原型，工作在 1060nm 波长，A 扫描率为 400kHz，用于对后眼部进行三维超高速成像。OCT 血管造影用于通过检测从视网膜上同一位置快速和重复获取的 OCT 强度横截面图像之间的红细胞运动对比，来对三维血管结构进行成像，而无需外源性荧光染料。通过光栅扫描获取横截面 OCT 血管造影体积，并在视网膜色素上皮（RPE）下方的多个深度对三维血管造影数据进行分割，从而可视化脉络膜毛细血管和脉络膜血管的面 OCT 血管造影。可以在不同的面深度可视化脉络膜毛细血管的精细微血管以及紧密排列的供养动脉和引流静脉网络。跨越视网膜约 32mm 的脉络膜毛细血管全景超宽视野拼接 OCT 血管造影显示出不同眼底位置的不同血管结构。在中央凹的较小区域和中央凹鼻侧约 6mm 的脉络膜毛细血管和 Sattler 层的较小区域，获得了孤立的视野，其血管结构与组织学和电子显微镜腐蚀铸造研究中建立的结构形态一致。对 8 名健康志愿者进行了脉络膜毛细血管成像，所有受试者均成功获得 OCT 血管造影。这些结果表明，超高速 OCT 具有在体无染料脉络膜毛细血管和脉络膜血管成像的可行性，除了常规的结构成像。
