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高密度聚合物微阵列：鉴定控制人类胚胎干细胞生长的合成聚合物。

High-density polymer microarrays: identifying synthetic polymers that control human embryonic stem cell growth.

机构信息

School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JJ, UK.

出版信息

Adv Healthc Mater. 2014 Jun;3(6):848-53. doi: 10.1002/adhm.201300489. Epub 2013 Dec 18.

DOI:10.1002/adhm.201300489
PMID:24353271
Abstract

The fabrication of high-density polymer microarray is described, allowing the simultaneous and efficient evaluation of more than 7000 different polymers in a single-cellular-based screen. These high-density polymer arrays are applied in the search for synthetic substrates for hESCs culture. Up-scaling of the identified hit polymers enables long-term cellular cultivation and promoted successful stem-cell maintenance.

摘要

本文描述了高密度聚合物微阵列的制作方法，该方法允许在单细胞筛选中同时高效地评估超过 7000 种不同的聚合物。这些高密度聚合物阵列被应用于寻找适合 hESCs 培养的合成基质。所鉴定的有效聚合物的放大化，使长期细胞培养和成功的干细胞维持成为可能。

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