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通过RNA集落印迹分析检测B细胞和前B细胞来源的集落中VH基因家族的利用情况。

VH gene family utilization in colonies derived from B and pre-B cells detected by the RNA colony blot assay.

作者信息

Wu G E, Paige C J

出版信息

EMBO J. 1986 Dec 20;5(13):3475-81. doi: 10.1002/j.1460-2075.1986.tb04672.x.

DOI:10.1002/j.1460-2075.1986.tb04672.x
PMID:2435544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1167383/
Abstract

The immunoglobulin heavy chain variable region (VH) genes of the mouse have been categorized into families based upon sequence homology. Utilizing the RNA colony blot assay we have determined the expression of eight of these families in B cell colonies derived from either surface immunoglobulin positive (sIg+) adult spleen B cells or sIg- fetal liver pre-B cells. We demonstrate, based upon the analysis of greater than 6000 individual colonies, that VH gene usage is a characteristic of the mouse strain studied. C57BL/6 mice most frequently (45%) utilize family VHJ558, the largest VH family, whereas BALB/c mice most frequently (22%) utilize family VH7183, the most JH proximal family in BALB/c mice. Moreover, colonies derived from sIg- fetal liver derived precursors show similar patterns, suggesting that selection based on exogenous antigen is not an important parameter in determining VH gene family usage.

摘要

小鼠免疫球蛋白重链可变区（VH）基因已根据序列同源性被归类为不同家族。利用RNA集落印迹分析，我们确定了这些家族中的8个在源自表面免疫球蛋白阳性（sIg+）成年脾B细胞或sIg-胎肝前B细胞的B细胞集落中的表达情况。基于对6000多个单个集落的分析，我们证明VH基因的使用情况是所研究小鼠品系的一个特征。C57BL/6小鼠最常（45%）使用最大的VH家族VHJ558，而BALB/c小鼠最常（22%）使用VH7183家族，这是BALB/c小鼠中最靠近JH的家族。此外，源自sIg-胎肝来源前体细胞的集落显示出相似的模式，这表明基于外源性抗原的选择在决定VH基因家族使用情况方面不是一个重要参数。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9ef/1167383/d65729b54bfd/emboj00176-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9ef/1167383/79e3485f3b57/emboj00176-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9ef/1167383/d65729b54bfd/emboj00176-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9ef/1167383/79e3485f3b57/emboj00176-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9ef/1167383/d65729b54bfd/emboj00176-0080-a.jpg

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Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.艾贝尔逊鼠白血病病毒转化的淋巴样细胞系中的多种免疫球蛋白重链基因转录物
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Differentiation and characterization of B-cell precursors detected in the yolk sac and embryo body of embryos beginning at the 10- to 12-somite stage.从10至12体节期开始，对在胚胎卵黄囊和胚体中检测到的B细胞前体进行分化和特征分析。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6429-33. doi: 10.1073/pnas.90.14.6429.
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Immunoglobulin variable gene segment VH81X of the mouse is embedded in L1 transposon sequences.小鼠的免疫球蛋白可变基因片段VH81X嵌入在L1转座子序列中。
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免疫球蛋白Cμ基因的转录本在淋巴细胞发育过程中结构和剪接方式存在差异。
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Diversity and joining segments of mouse immunoglobulin heavy chain genes are closely linked and in the same orientation: implications for the joining mechanism.小鼠免疫球蛋白重链基因的多样性和连接片段紧密相连且方向相同：对连接机制的启示。
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