VH基因家族的利用受VH区域之外的一个基因座调控。
VH gene family utilization is regulated by a locus outside of the VH region.
作者信息
Wu G E, Paige C J
机构信息
Basel Institute for Immunology, Switzerland.
出版信息
J Exp Med. 1988 Apr 1;167(4):1499-504. doi: 10.1084/jem.167.4.1499.
Abstract
We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain-specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus.
摘要
我们使用RNA集落印迹法检测了源自原代脾B细胞的集落中的VH使用情况。我们发现VH使用模式存在品系特异性差异。利用亲代F1、同源近交系和重组近交系，我们证明导致观察到的表型的遗传元件具有以下特点：(a)稳定表达；(b)非母源影响所致；(c)非显性可扩散因子所致；(d)与克隆效率无关；(e)位于Igh基因座之外。
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