McKetta Department of Chemical Engineering; University of Texas at Austin; Austin, TX USA.
RNA Biol. 2013 Dec;10(12):1778-97. doi: 10.4161/rna.27102. Epub 2013 Nov 18.
RNAs have many important functional properties, including that they are independently controllable and highly tunable. As a result of these advantageous properties, their use in a myriad of sophisticated devices has been widely explored. Yet, the exploitation of RNAs for synthetic applications is highly dependent on the ability to characterize the many new molecules that continue to be discovered by large-scale sequencing and high-throughput screening techniques. In this review, we present an exhaustive survey of the most recent synthetic bacterial riboswitches and small RNAs while emphasizing their virtues in gene expression management. We also explore the use of these RNA components as building blocks in the RNA synthetic biology toolbox and discuss examples of synthetic RNA components used to rewire bacterial regulatory circuitry. We anticipate that this field will expand its catalog of smart devices by mimicking and manipulating natural RNA mechanisms and functions.
RNAs 具有许多重要的功能特性，包括它们具有独立性和高度可调性。由于这些有利的特性，人们广泛探索了在众多复杂设备中使用 RNA。然而，RNA 在合成应用中的利用高度依赖于对通过大规模测序和高通量筛选技术不断发现的许多新分子进行表征的能力。在这篇综述中，我们全面介绍了最近的合成细菌核糖开关和小 RNA，并强调了它们在基因表达管理方面的优点。我们还探讨了这些 RNA 组件在 RNA 合成生物学工具包中的构建模块的用途，并讨论了用于重新连接细菌调控回路的合成 RNA 组件的示例。我们预计，该领域将通过模拟和操纵天然 RNA 机制和功能来扩展其智能设备目录。
