Andersen J, Delihas N, Ikenaka K, Green P J, Pines O, Ilercil O, Inouye M
Nucleic Acids Res. 1987 Mar 11;15(5):2089-101. doi: 10.1093/nar/15.5.2089.
A new species of micF RNA, which contains 93 nucleotides (a 4.5S size), was isolated from Escherichia coli. The sequence of the 4.5S micF RNA corresponds to positions G82 through U174 of the micF gene. The 5' terminal end of this smaller micF RNA is triphosphorylated signifying that it is a primary transcript. Its promoter region, which is situated within the greater micF structural gene, has been identified and characterized by lacZ fusion analysis. A 6S micF RNA species, which has a base composition predicted for a transcript from the full length gene has also been detected; however, the 4.5S micF RNA is the predominant species. The work clearly shows by biochemical identification the presence of chromosomally encoded micF RNA.
从大肠杆菌中分离出一种新的micF RNA物种，它含有93个核苷酸（4.5S大小）。4.5S micF RNA的序列对应于micF基因的G82至U174位置。这种较小的micF RNA的5'末端是三磷酸化的，这表明它是一个初级转录本。其启动子区域位于更大的micF结构基因内，已通过lacZ融合分析进行了鉴定和表征。还检测到了一种6S micF RNA物种，其碱基组成与全长基因转录本的预测相符；然而，4.5S micF RNA是主要物种。这项工作通过生化鉴定清楚地表明了染色体编码的micF RNA的存在。
