Environmental Virology Cell, Council for Scientific and Industrial Research - National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440020, Maharashtra, India.
BMC Vet Res. 2013 Dec 26;9:264. doi: 10.1186/1746-6148-9-264.
The bacteriophage therapy is an effective antimicrobial approach with potentially important applications in medicine and biotechnology which can be seen as an additional string in the bow. Emerging drug resistant bacteria in aquaculture industry due to unrestricted use of antibiotics warrants more sustainable and environmental friendly strategies for controlling fish infections.The isolated bacteria from fish lesions was characterised based on isolation on selective and differential medium like Pseudomonas agar, gram staining, biochemical tests and 16SrRNA sequencing. The metallo-beta-lactamase (MBL) producing bacterial isolate was evaluated using Imipenem - Ethylenediaminetetraacetic acid (EDTA) disk method. The specific bacteriophage was isolated and concentrated using coal bed developed in our lab at CSIR-NEERI. The isolated and enriched bacteriophage was characterised by nucleotide sequencing and electron microscopy. The phage therapy was applied for treating ulcerative lesion in fish.
The pathogenic bacterium responsible for causing ulcerative lesions in catfish species (Clarias gariepinus) was identified as Pseudomonas aeruginosa. One out of twenty P. aeruginosa isolate showing multi drug resistance (MDR) was incidentally found to be MBL producing as determined by Imipenem-EDTA disk method. The phage therapy effectively cured the ulcerative lesions of the infected fish in 8-10 days of treatment, with a sevenfold reduction of the lesion with untreated infection control.
Bacteriophage therapy can have potential applications soon as an alternative or as a complement to antibiotic treatment in the aquaculture. We present bacteriophage therapy as a treatment method for controlling MDR P. aeruginosa infection in C. gariepinus. To the best of our knowledge this is a first report of application of phage therapy against MBL producing P. aeruginosa isolated from aquatic ecosystem.
噬菌体疗法是一种有效的抗菌方法，在医学和生物技术方面具有重要的潜在应用，可以被视为一种额外的手段。由于抗生素的无限制使用，水产养殖行业中出现了耐药细菌，因此需要更可持续和环保的策略来控制鱼类感染。从鱼类病变中分离出的细菌根据在选择性和差异化培养基（如假单胞菌琼脂、革兰氏染色、生化试验和 16SrRNA 测序）上的分离特性进行了鉴定。采用亚胺培南-乙二胺四乙酸（EDTA）纸片法对产金属β-内酰胺酶（MBL）的细菌分离株进行了评估。使用我们在 CSIR-NEERI 的实验室开发的煤层从鱼病组织中分离和浓缩了特异性噬菌体。通过核苷酸测序和电子显微镜对分离和富集的噬菌体进行了鉴定。噬菌体疗法用于治疗鱼类溃疡性病变。
引起鲶鱼（Clarias gariepinus）溃疡性病变的病原菌被鉴定为铜绿假单胞菌。在 20 株铜绿假单胞菌分离株中，有一株偶然被发现是 MBL 产生菌，这是通过亚胺培南-EDTA 纸片法确定的。噬菌体疗法在 8-10 天的治疗后有效地治愈了受感染鱼类的溃疡性病变，与未治疗的感染对照组相比，病变减少了七倍。
噬菌体疗法很快就有可能作为抗生素治疗的替代或补充方法在水产养殖中得到应用。我们提出噬菌体疗法作为控制 MDR 铜绿假单胞菌感染的治疗方法。据我们所知，这是首次报道在水生生态系统中分离出的产 MBL 铜绿假单胞菌应用噬菌体疗法。
