日本广岛耐亚胺培南铜绿假单胞菌的分子特征
Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan.
作者信息
Ohara Masaru, Kouda Shuntaro, Onodera Makoto, Fujiue Yoshihiro, Sasaki Megumi, Kohara Tadahiro, Kashiyama Seiya, Hayashida Shizue, Kadono Manami, Komatsuzawa Hitoshi, Gotoh Naomasa, Usui Tuguru, Itaha Hideyuki, Kuwabara Masao, Yokoyama Takashi, Sugai Motoyuki
机构信息
Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
出版信息
Microbiol Immunol. 2007;51(3):271-7. doi: 10.1111/j.1348-0421.2007.tb03908.x.
Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-beta-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing P. aeruginosa strains in Japanese hospitals.
在日本广岛市6家医院（a - f）的1058株菌株中，发现100株（9.5%）铜绿假单胞菌对亚胺培南耐药。在这100株菌株中，14株（14%）使用巯基乙酸钠纸片进行双纸片协同试验呈阳性，18株（18%）通过聚合酶链反应（PCR）检测bla(IMP - 1)或bla(VIM - 2)等位基因呈阳性。在100株亚胺培南耐药菌株中，32株被归类为多重耐药菌株，其中13株金属β - 内酰胺酶基因呈阳性。100株亚胺培南耐药菌株中有51株（51%）对左氧氟沙星的RND外排泵活性升高。但51株中只有6株被归类为多重耐药菌株。对这100株分离株经Spe I酶切的DNA进行脉冲场凝胶电泳分析表明，不仅存在克隆传播，而且异质克隆的传播开始促使产金属β - 内酰胺酶的铜绿假单胞菌菌株在日本医院中流行。
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