IFITM3 在人神经胶质瘤细胞生长和迁移中的作用。
The role of IFITM3 in the growth and migration of human glioma cells.
机构信息
Department of Neurosurgery, the Second Affiliated Hospital of Anhui Medical University, 678 Fu Rong Road, Hefei 230601, China.
出版信息
BMC Neurol. 2013 Dec 27;13:210. doi: 10.1186/1471-2377-13-210.
BACKGROUND
Interferon induced transmembrane protein 3 (IFITM3) is transcribed in most tissues and highly interferon-inducible. However, the role of IFITM3 in cancer is still poorly understood.
METHODS
Expression levels of IFITM3 were analyzed in 60 glioma patients by immunohistochemistry (IHC). Following closely, we investigated the phenotype of IFITM3 knockdown on glioma cell growth and tumorigenesis in vitro using lentivirus-mediated loss-of-function strategy.
RESULTS
Depletion of IFITM3in U251 cells dramatically inhibited cell proliferation and colony formation, which demonstrated that reduced IFITM3 protein levels could cause inhibition of tumorigenesis. Knockdown of IFITM3 also induced cell cycle arrest in G0/G1 phase, especially in the sub-G1 phase representing apoptotic cells. In addition, the migration of U251 cells was visibly weakened after IFITM3 knockdown, as determined by Transwell assay.
CONCLUSIONS
Our findings provide new evidence that IFITM3 plays an important role in glioma cell growth and migration, suggesting that silencing of IFITM3 by RNA interference (RNAi) may be a potential approach to suppress glioma growth.
背景
干扰素诱导跨膜蛋白 3（IFITM3）在大多数组织中均有转录，并且高度诱导干扰素。然而，IFITM3 在癌症中的作用仍知之甚少。
方法
通过免疫组织化学（IHC）分析 60 名胶质瘤患者的 IFITM3 表达水平。随后，我们使用慢病毒介导的功能丧失策略，研究了 IFITM3 敲低对体外胶质瘤细胞生长和致瘤性的表型。
结果
IFITM3 在 U251 细胞中的耗竭显着抑制细胞增殖和集落形成，这表明减少 IFITM3 蛋白水平可抑制肿瘤发生。IFITM3 的敲低还诱导细胞周期停滞在 G0/G1 期，特别是在亚 G1 期代表凋亡细胞。此外，通过 Transwell 测定，IFITM3 敲低后 U251 细胞的迁移明显减弱。
结论
我们的研究结果提供了新的证据，表明 IFITM3 在神经胶质瘤细胞生长和迁移中发挥重要作用，提示通过 RNA 干扰（RNAi）沉默 IFITM3 可能是抑制神经胶质瘤生长的一种潜在方法。
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