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用单克隆抗体免疫金染色法对沙眼衣原体表面抗原进行超微结构研究。

Ultrastructural study of Chlamydia trachomatis surface antigens by immunogold staining with monoclonal antibodies.

作者信息

Kuo C C, Chi E Y

出版信息

Infect Immun. 1987 May;55(5):1324-8. doi: 10.1128/iai.55.5.1324-1328.1987.

DOI:10.1128/iai.55.5.1324-1328.1987
PMID:2437035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260509/
Abstract

Surface antigens of Chlamydia trachomatis were studied by immunogold staining with monoclonal antibodies and by electron microscopy. The serovar- and subspecies-specific epitopes were the most surface accessible. The species- and genus-specific epitopes were the least surface exposed. Similar serological specificity as that in the microimmunofluorescence test was demonstrated by immunogold staining.

摘要

采用单克隆抗体免疫金染色及电子显微镜技术对沙眼衣原体的表面抗原进行了研究。血清型和亚种特异性表位最易暴露于表面。种属特异性表位最不易暴露于表面。免疫金染色显示出与微量免疫荧光试验相似的血清学特异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2014/260509/cfa8c47b2713/iai00089-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2014/260509/59d84de0b170/iai00089-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2014/260509/cfa8c47b2713/iai00089-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2014/260509/59d84de0b170/iai00089-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2014/260509/cfa8c47b2713/iai00089-0316-a.jpg

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本文引用的文献

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Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.抗沙眼衣原体单克隆抗体：抗体特异性及抗原特性
J Immunol. 1982 Mar;128(3):1083-9.
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Ultrastructure of mast cell degranulation induced by eosinophil peroxidase: Use of diaminobenzidine cytochemistry by scanning electron microscopy.
单克隆抗体与反刍兽考德里氏体表面暴露的一个表位结合，该表位在八个菌株中是保守的。
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Topological analysis of Chlamydia trachomatis L2 outer membrane protein 2.沙眼衣原体L2外膜蛋白2的拓扑分析
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