Department of Endocrinology and Metabolism, Firat University Hospital, 23119 Elazig, Turkey.
Department of Medical Biochemistry and Clinical Biochemistry, Firat Hormones Research Group, Firat University Hospital, 23119 Elazig, Turkey.
Int J Endocrinol. 2013;2013:236085. doi: 10.1155/2013/236085. Epub 2013 Nov 25.
This study examines the levels of acylated and desacylated ghrelin, preptin, leptin, and nesfatin-1 peptide changes related to the body mass index (BMI). The subjects were allocated to 5 groups depending on their BMIs as follows: Group I (BMI <18.5 kg/m(2)); Group II (BMI 18.5-24.9 kg/m(2)); Group III (BMI 25-29.9 kg/m(2)); Group IV (BMI 30-39.9 kg/m(2)); Group V (BMI >40 kg/m(2)). Serum acylated and desacylated ghrelin, preptin, and leptin levels were measured by the enzyme-linked immunosorbent assay (ELISA) and nesfatin-1 was measured by the enzyme immunoassay (EIA). Desacylated ghrelin levels showed a gradual and statistically significant drop from Group I to Group V, while preptin and leptin levels exhibited a gradual and significant increase from Group I to Group IV. Serum nesfatin-1 levels gradually, but not significantly, increased from Group I to Group III and showed a significant decrease in Groups IV and V. In conclusion, leptin, preptin, and acylated ghrelin (AG) levels increased with higher BMI, whereas desacylated ghrelin (DAG) decreased and nesfatin-1 showed no clear relationship to BMI.
本研究旨在探讨体质量指数（BMI）与酰化和去酰化 ghrelin、前蛋白、瘦素和 nesfatin-1 肽水平变化的关系。将受试者根据 BMI 分为 5 组：I 组（BMI<18.5kg/m²）；II 组（BMI 18.5-24.9kg/m²）；III 组（BMI 25-29.9kg/m²）；IV 组（BMI 30-39.9kg/m²）；V 组（BMI>40kg/m²）。采用酶联免疫吸附试验（ELISA）检测血清酰化和去酰化 ghrelin、前蛋白和瘦素水平，采用酶免疫分析法（EIA）检测 nesfatin-1 水平。结果显示，去酰化 ghrelin 水平自 I 组至 V 组逐渐下降，且差异有统计学意义，而前蛋白和瘦素水平自 I 组至 IV 组逐渐升高。血清 nesfatin-1 水平自 I 组至 III 组逐渐升高，但差异无统计学意义，自 IV 组至 V 组则明显降低。结论：随着 BMI 的增加，瘦素、前蛋白和酰化 ghrelin（AG）水平升高，而去酰化 ghrelin（DAG）水平降低，nesfatin-1 与 BMI 无明显关系。
