Magalhães A C, Levy F M, Souza B M, Cardoso C A B, Cassiano L P, Pessan J P, Buzalaf M A R
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
J Dent. 2014 Apr;42(4):498-502. doi: 10.1016/j.jdent.2013.12.009. Epub 2013 Dec 25.
This in vitro study assessed the effect of milk containing different fluoride concentrations on tooth erosion.
Bovine enamel and root dentine specimens were treated with: (1) bovine whole milk with 0ppm F; (2) 2.5ppm F; (3) 5ppm F; (4) 10ppm F (all after erosion); (5) whole milk with 0ppm F (before erosion); (6) NaF (0.05% F, positive control, after erosion) or (7) 0.9% NaCl (negative control, after erosion). The specimens were submitted to pH cycles (4× 90s in soft drink) and treatments for 5 days. The specimens were immersed in the treatment solutions for 1min (only at the first cycle each day) with further exposition to 1:1 milk:saliva slurry for 10min. The tooth loss was measured using a contact profilometer and statistically analysed (p<0.05).
Rinsing with milk before erosive challenge significantly reduced tooth loss compared to negative control (67% and 24% reduction in dentine and enamel loss, respectively) and to milk after erosive challenge, only for dentine. The addition of fluoride to milk also reduced tooth loss compared to negative control, but with no significant differences among fluoride concentrations for enamel and dentine (μm), respectively: 0ppm (3.63±0.04 and 2.51±0.53), 2.5ppm F (2.86±0.42 and 1.96±0.47), 5ppm F (2.81±0.27 and 1.77±0.44), 10ppm F (2.03±0.49 and 1.68±0.59). There was a negative and significant correlation between [F] and the tooth loss.
Daily rinse with milk containing F is able to reduce both enamel and dentine erosion in vitro.
Since the prevalence of dental erosion is steadily increasing, rinse with milk or its derivate might be an important strategy to reduce the progression of tooth erosion.
本体外研究评估了含不同氟浓度的牛奶对牙齿侵蚀的影响。
牛牙釉质和牙根牙本质标本接受以下处理：（1）含0ppm氟的全脂牛奶；（2）含2.5ppm氟的全脂牛奶；（3）含5ppm氟的全脂牛奶；（4）含10ppm氟的全脂牛奶（均在侵蚀后）；（5）含0ppm氟的全脂牛奶（在侵蚀前）；（6）氟化钠（0.05%氟，阳性对照，侵蚀后）或（7）0.9%氯化钠（阴性对照，侵蚀后）。标本进行pH循环（在软饮料中4×90秒）并处理5天。标本每天仅在第一个循环时浸入处理溶液1分钟，然后再暴露于1:1的牛奶：唾液浆液中10分钟。使用接触式轮廓仪测量牙齿损失并进行统计学分析（p<0.05）。
与阴性对照相比，在侵蚀性刺激前用牛奶漱口可显著减少牙齿损失（牙本质和牙釉质损失分别减少67%和24%），且仅对于牙本质而言，与侵蚀性刺激后用牛奶漱口相比也有减少。与阴性对照相比，向牛奶中添加氟也可减少牙齿损失，但牙釉质和牙本质在不同氟浓度之间无显著差异（μm），分别为：0ppm（3.63±0.04和2.51±0.53）、2.5ppm氟（2.86±0.42和1.96±0.47）、5ppm氟（2.81±0.27和1.77±0.44）、10ppm氟（2.03±0.49和1.68±0.59）。[氟]与牙齿损失之间存在负相关且具有显著性。
每天用含氟牛奶漱口能够在体外减少牙釉质和牙本质的侵蚀。
由于牙齿侵蚀的患病率在稳步上升，用牛奶或其衍生物漱口可能是减少牙齿侵蚀进展的重要策略。
