Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Texas.
J Clin Lab Anal. 2014 Jan;28(1):16-20. doi: 10.1002/jcla.21637. Epub 2013 Dec 27.
Oleander interferes with serum digoxin measurements using various immunoassays. The potential interference of oleander and its active ingredient, oleandrin, with a relatively new homogenous sequential chemiluminescent digoxin assay based on luminescent oxygen channeling technology (LOCI digoxin assay, Siemens Diagnostics) has not been previously reported.
Aliquots of a digoxin-free serum pool were supplemented with increasing concentrations of oleandrin, or with oleander extract, followed by measuring the apparent digoxin concentrations using the LOCI digoxin assay using Vista 1500 analyzer. Mice were fed oleandrin or oleander extract, and their blood digoxin levels at 1 and 2 h were measured with the LOCI digoxin assay. In addition, two digoxin serum pools were prepared by combining sera of patients receiving digoxin; aliquots of both pools were supplemented with oleandrin or oleander extract and digoxin concentrations were again measured. Attempts to overcome this interference were made by measuring free digoxin concentration using a third digoxin pool.
Significant apparent digoxin concentrations were observed after supplementing aliquots of the drug-free serum pool with oleandrin or oleander extract. Mice fed with oleandrin or oleander extract also showed apparent digoxin levels 1 and 2 h after feeding. Digoxin values were also falsely lower or elevated (bidirectional interference) when aliquots of digoxin serum pools were further supplemented with oleandrin or oleander extract depending on concentration; this interference was not eliminated by free digoxin monitoring.
Oleandrin interferes with LOCI digoxin assay.
夹竹桃会干扰使用各种免疫测定法的血清地高辛测量。夹竹桃及其活性成分欧夹竹桃苷与一种基于发光氧通道技术（LOCI 地高辛测定法，西门子诊断公司）的相对较新的均相连续化学发光地高辛测定法之间的潜在干扰尚未有报道。
在无地高辛血清池的等分试样中添加浓度逐渐增加的欧夹竹桃苷或夹竹桃提取物，然后使用 Vista 1500 分析仪上的 LOCI 地高辛测定法测量表观地高辛浓度。用欧夹竹桃苷或夹竹桃提取物喂养小鼠，并用 LOCI 地高辛测定法测量其 1 和 2 小时时的血液地高辛水平。此外，通过合并接受地高辛治疗的患者的血清来制备两个地高辛血清池；将两个池的等分试样分别用欧夹竹桃苷或夹竹桃提取物补充，并再次测量地高辛浓度。通过使用第三个地高辛池测量游离地高辛浓度来尝试克服这种干扰。
在用欧夹竹桃苷或夹竹桃提取物补充无药血清池的等分试样后，观察到明显的地高辛浓度。用欧夹竹桃苷或夹竹桃提取物喂养的小鼠在喂养后 1 和 2 小时也显示出明显的地高辛水平。根据浓度，进一步向地高辛血清池的等分试样中添加欧夹竹桃苷或夹竹桃提取物，也会导致地高辛值偏低或偏高（双向干扰）；通过游离地高辛监测无法消除这种干扰。
欧夹竹桃苷会干扰 LOCI 地高辛测定法。
