Harisa Gamaleldin I
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Biochemistry, College of Pharmacy, Al-Azhar University (Boys), Nasr City, Cairo, Egypt.
J Biochem Mol Toxicol. 2014 Mar;28(3):129-36. doi: 10.1002/jbt.21544. Epub 2013 Dec 27.
In this study, the protective role of naringin (NAR) against paclitaxel (PTX)-induced erythrocytes aging has been investigated using human erythrocyte as an in vitro model. Erythrocytes were incubated with PTX in the presence and absence of NAR. Incubation of erythrocytes with PTX resulted in increased protein carbonyl content and malondialdehyde and hemolysis percentage compared with control. Furthermore, a significant increase in the ratios of glutathione peroxidase/glutathione reductase, superoxide dismutase/glutathione peroxidase, and superoxide dismutase/catalase in PTX-treated cells was observed, compared with control cells. In contrast, reduced glutathione/oxidized glutathione ratio and glucose-6-phosphate dehydrogenase activity were decreased upon PTX treatment. The simultaneous incubation of erythrocytes with PTX and NAR restored these variables to values similar to those of control erythrocytes. These results suggest that NAR inhibited PTX-induced aging by lessening the PTX-induced oxidative stress.
在本研究中，以人红细胞作为体外模型，研究了柚皮苷（NAR）对紫杉醇（PTX）诱导的红细胞衰老的保护作用。在有和没有NAR的情况下，将红细胞与PTX一起孵育。与对照组相比，PTX孵育红细胞导致蛋白质羰基含量、丙二醛和溶血百分比增加。此外，与对照细胞相比，在PTX处理的细胞中，谷胱甘肽过氧化物酶/谷胱甘肽还原酶、超氧化物歧化酶/谷胱甘肽过氧化物酶和超氧化物歧化酶/过氧化氢酶的比率显著增加。相反，PTX处理后，还原型谷胱甘肽/氧化型谷胱甘肽比率和葡萄糖-6-磷酸脱氢酶活性降低。红细胞与PTX和NAR同时孵育可将这些变量恢复到与对照红细胞相似的值。这些结果表明，NAR通过减轻PTX诱导的氧化应激来抑制PTX诱导的衰老。
