Coleman J L, Benach J L, Beck G, Habicht G S
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):123-6. doi: 10.1016/s0176-6724(86)80112-2.
Borrelia burgdorferi consists of an inner protoplasmic cylinder, containing the genome and cytoplasmic elements, surrounded by a number of axial filaments, all completely encased within a multiple-layered outer envelope structure (OE). In this study, a sodium dodecyl sulfate-mediated technique was used to isolate the OE from Borrelia burgdorferi in an attempt to better understand this structure in terms of its antigenic reactivity to Lyme disease patient sera. Electron microscopic evidence suggested that the OE product was relatively free of other spirochete cellular components. SDS-PAGE analysis indicated that the electrophoretic pattern of the OE was consistent with that of the remaining protoplasmic cylinder (PC) and whole spirochete controls. Antigenic determinants in the OE were recognized by sera from Lyme disease patients in Western blots. Chemical analysis of the OE revealed a composition of 45.90% protein, 50.75% lipid and 3.33% carbohydrate. The OE comprised 16.5% by lyophilized dry weight of the whole spirochete. Antigenic determinants located within or associated with this structure are likely to play a significant role in the development of immunity in the infected host.
伯氏疏螺旋体由一个内部原生质圆柱体组成，包含基因组和细胞质成分，周围环绕着许多轴丝，所有这些都完全包裹在一个多层的外膜结构（OE）内。在本研究中，使用十二烷基硫酸钠介导的技术从伯氏疏螺旋体中分离出OE，试图从其对莱姆病患者血清的抗原反应性方面更好地了解该结构。电子显微镜证据表明，OE产物相对不含其他螺旋体细胞成分。SDS-PAGE分析表明，OE的电泳图谱与其余原生质圆柱体（PC）和整个螺旋体对照的电泳图谱一致。在蛋白质免疫印迹中，莱姆病患者的血清识别出OE中的抗原决定簇。对OE的化学分析显示，其组成为45.90%的蛋白质、50.75%的脂质和3.33%的碳水化合物。OE占整个螺旋体冻干干重的16.5%。位于该结构内或与之相关的抗原决定簇可能在感染宿主的免疫发展中起重要作用。
