聚酰胺-胺型树枝状大分子增强姜黄素对癌细胞增殖的抑制作用：可能对端粒酶有抑制作用。
PAMAM dendrimers augment inhibitory effects of curcumin on cancer cell proliferation: possible inhibition of telomerase.
作者信息
Mollazade Mahdie, Nejati-Koshki Kazem, Akbarzadeh Abolfazl, Zarghami Nosratollah, Nasiri Marzieh, Jahanban-Esfahlan Rana, Alibakhshi Abbas
机构信息
Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail :
出版信息
Asian Pac J Cancer Prev. 2013;14(11):6925-8. doi: 10.7314/apjcp.2013.14.11.6925.
BACKGROUND
Despite numerous useful anticancer properties of curcumin, its utility is limited due to its hydrophobic structure. In this study, we investigated the comparative antiproliferative effect of PAMAM encapsulating curcumin with naked curcumin on the T47D breast cancer cell line.
MATERIALS AND METHODS
Cytotoxic effects of PAMAM dendrimers encapsulating curcumin and curcumin alone were investigated by MTT assay. After treating cells with different concentrations of both curcumin alone and curcumin encapsulated for 24h, telomerase activity was determined by TRAP assay.
RESULTS
While PAMAM dendrimers encapsulating curcumin had no cytotoxicity on cancer cells, they decreased the IC50 for proliferation and also increased the inhibitory effect on telomerase activity.
CONCLUSIONS
Considering the non-toxicity in addition to effectiveness for enhancing curcumin anticancer properties, dendrimers could be considered good therapeutic vehicles for this hydrophobic agent.
背景
尽管姜黄素具有多种有益的抗癌特性，但其疏水性结构限制了其效用。在本研究中，我们研究了包裹姜黄素的聚酰胺 - 胺型树枝状大分子（PAMAM）与游离姜黄素对T47D乳腺癌细胞系的增殖抑制作用比较。
材料与方法
采用MTT法研究包裹姜黄素的PAMAM树枝状大分子和姜黄素单独使用时的细胞毒性作用。用不同浓度的游离姜黄素和包裹姜黄素处理细胞24小时后，通过端粒重复扩增法（TRAP）测定端粒酶活性。
结果
包裹姜黄素的PAMAM树枝状大分子对癌细胞无细胞毒性，但它们降低了增殖的半数抑制浓度（IC50），并增强了对端粒酶活性的抑制作用。
结论
考虑到树枝状大分子在增强姜黄素抗癌特性方面有效且无毒，可将其视为这种疏水性药物的良好治疗载体。
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