Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P. R. China.
J Microbiol. 2013 Dec;51(6):777-82. doi: 10.1007/s12275-013-3279-2. Epub 2013 Dec 19.
Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 10(10) CFU of LC01 each day for the 4-week treatment period, and then consumed placebo in the next treatment period, separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichia coli and increase in Lactobacillus, Bifidobacterium, and Roseburia intestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.
副干酪乳杆菌亚种副干酪乳杆菌 LC01（LC01）能够耐受肠道应激，具有抗氧化活性。为了评估该细菌对人体肠道微生物群的影响，进行了一项随机、双盲、安慰剂对照的人体试验。将 52 名健康成年志愿者随机平均分为两组。一组每天食用 12%（wt/vol）脱脂奶，补充 10（10）CFU 的 LC01，持续 4 周治疗期，然后在下一个治疗期食用安慰剂，间隔 2 周洗脱期。另一组则按照相反的顺序进行。使用组特异性实时 PCR 和生化分析来确定每个时期结束时收集的粪便样本中的肠道细菌组成，以及短链脂肪酸和氨的浓度。在食用 LC01 后，粪便中的大肠杆菌明显受到抑制，而乳杆菌、双歧杆菌和罗斯伯里亚肠道菌的数量增加。乙酸和丁酸在益生菌阶段明显更高，粪便中的氨明显更低。结果表明 LC01 对年轻人的肠道微生物群具有调节作用，提示对肠道健康有益。LC01 可能作为益生菌具有潜在价值。
