Tak V, Mirdha B R, Yadav P, Vyas P, Makharia G K, Bhatnagar S
Department of Microbiology, AIIMS, New Delhi, India.
Indian J Med Microbiol. 2014 Jan-Mar;32(1):19-25. doi: 10.4103/0255-0857.124290.
The aim of the study was to determine the genetic heterogeneity of Giardia intestinalis isolates detected in stool samples of the study population using polymerase chain reaction assay and restriction fragment length polymorphism. We also tried to correlate the association/differences between the clinical symptomatology and infection by different assemblages (genotypes) of G. intestinalis.
This cross-sectional study was conducted from April 2008 to June 2010. A total of 40 adults (n = 40) and 42 children (n = 42) below the age of 12 years with the clinical suspicion of giardiasis and with the onset of one or more of the following five symptoms, i.e., loose stool, nausea, weight loss, fatigue and foul smelling faeces and confirmed laboratory diagnosis of giardiasis at least once during the current episode of diarrhoea were included in this study.
Of the 82 patients (males 66) enrolled in the study, 70 (85%) presented with diarrhoea (56 males) and 12 (15%) without diarrhoea (10 males). Out of 70 diarrheic patients, 61 (87%) had chronic diarrhoea, 8 (11.5%) had acute diarrhoea and 1 (1.5%) had persistent diarrhoea. Of the total patients, 63 (77%) were clinically assessed and were apparently immunocompetent, whereas, 19 (23%) immunocompromised patients had different underlying conditions besides giardiasis. Genotyping identified all 82 (100%) isolates as assemblage B.
We found that assemblage B of G. intestinalis presents with all kinds of clinical features ranging from asymptomatic carriage to acute, persistent or chronic diarrhoea.
本研究旨在利用聚合酶链反应检测和限制性片段长度多态性，确定在研究人群粪便样本中检测到的肠道贾第虫分离株的基因异质性。我们还试图关联肠道贾第虫不同组合（基因型）感染与临床症状之间的关联/差异。
本横断面研究于2008年4月至2010年6月进行。纳入了40名成年人（n = 40）和42名12岁以下儿童（n = 42），他们临床上疑似患有贾第虫病，且出现以下五种症状中的一种或多种，即腹泻、恶心、体重减轻、疲劳和粪便恶臭，并且在当前腹泻发作期间至少有一次经实验室确诊为贾第虫病。
在纳入研究的82名患者（男性66名）中，70名（85%）出现腹泻（男性56名），12名（15%）未出现腹泻（男性10名）。在70名腹泻患者中，61名（87%）患有慢性腹泻，8名（11.5%）患有急性腹泻，1名（1.5%）患有持续性腹泻。在所有患者中，63名（77%）经临床评估具有明显的免疫能力，而19名（23%）免疫功能低下的患者除贾第虫病外还有其他基础疾病。基因分型确定所有82株（100%）分离株均为B组。
我们发现肠道贾第虫B组呈现出从无症状携带到急性、持续性或慢性腹泻的各种临床特征。
