Darah I, Lim S H, Nithianantham K
Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Indian J Pharm Sci. 2013 Sep;75(5):533-9.
The antibacterial activity of the methanol extract of Wedelia chinensis leave was studied and tested against three pathogenic Gram positive bacteria (Bacillus cereus, B. subtilis and Stapylococcus aureus) and three pathogenic Gram negative bacteria (Escherichia coli, Proteus rettgeri and Pseudomonas aeruginosa) by the disk diffusion assay and broth dilution methods. The extract exhibited favourable antibacterial activity against the bacterial cells but was more potent against Gram positive bacteria with the minimum inhibition concentration of 3.12 to 6.25 mg/ml compared to the Gram negative bacteria which had minimum inhibition concentration values of 25 mg/ml. The time-kill study suggested that the extract possessed bactericidal properties at higher concentrations and eradicated the growth of bacterial cells. The major abnormalities occurred to the bacterial cells after exposed to the extract were complete alterations in their morphology and collapsed of the cells beyond repair. The methanol extract of W. chinensis may be an effective antibacterial agent to treat bacterial infections.
研究了蟛蜞菊叶甲醇提取物对三种革兰氏阳性病原菌（蜡样芽孢杆菌、枯草芽孢杆菌和金黄色葡萄球菌）以及三种革兰氏阴性病原菌（大肠杆菌、雷氏变形杆菌和铜绿假单胞菌）的抗菌活性，并采用纸片扩散法和肉汤稀释法进行测试。该提取物对细菌细胞表现出良好的抗菌活性，但对革兰氏阳性菌的作用更强，其最低抑菌浓度为3.12至6.25毫克/毫升，而革兰氏阴性菌的最低抑菌浓度值为25毫克/毫升。时间-杀菌研究表明，该提取物在较高浓度下具有杀菌特性，可消除细菌细胞的生长。暴露于提取物后，细菌细胞出现的主要异常情况是其形态完全改变且细胞崩溃无法修复。蟛蜞菊甲醇提取物可能是一种治疗细菌感染的有效抗菌剂。
