Jang Yo-Soon, Kim Ki-Yong, Kim Sung
a East Sea Environment Research Division, Korea Institute of Ocean Science & Technology , 695-1, Hujung-ri, Jukbyeon-myun, Uljin-gun , Gyeongbuk , Republic of Korea .
b Department of Marine Biotechnology , University of Science & Technology , 217 Gajungro Yuseong-gu , Daejeon , Republic of Korea .
Mitochondrial DNA. 2015;26(6):834-5. doi: 10.3109/19401736.2013.855916. Epub 2014 Jan 10.
The complete mitochondrial genome sequence of Sebastes koreanus was determined by next-generation sequencing. The total genome was 16,499 bp in length, comprising two rRNA, 22 tRNA and 13 protein-coding genes (PCGs), as well as the mtDNA control region. The genome constitutes 27.9% A, 26.6% T, 17.1% G and 28.4% C, showing a slight AT bias (54.5%). Of the 13 mitochondrial PCGs, 12 have an ATG start codon while COX1 had a GTG start codon; the incomplete stop codon (T) was present in the ND3, ND4 and Cytb genes. The anti-G bias was considerably lower at the third codon position of PCGs (8.8%). The complete mitogenome sequence of S. koreanus will contribute to molecular phylogenetic and evolutionary analyses of Sebastinae and to the identification of its genetic populations in the Yellow Sea.
通过下一代测序确定了朝鲜平鲉的完整线粒体基因组序列。该基因组全长16,499 bp,包括两个rRNA、22个tRNA和13个蛋白质编码基因(PCGs),以及线粒体DNA控制区。基因组中A占27.9%,T占26.6%,G占17.1%,C占28.4%,显示出轻微的AT偏向性(54.5%)。在13个线粒体PCGs中,12个具有ATG起始密码子,而COX1具有GTG起始密码子;ND3、ND4和Cytb基因中存在不完全终止密码子(T)。PCGs第三个密码子位置的抗G偏向性相当低(8.8%)。朝鲜平鲉的完整线粒体基因组序列将有助于平鲉亚科的分子系统发育和进化分析,以及黄海其遗传种群的鉴定。