Jang Yo-Soon, Kim Ki-Yong, Oh Sung-Yong, Choi Hee-Jung, Myoung Jung-Goo, Kim Sung
a East Sea Environment Research Division , Korea Institute of Ocean Science & Technology , Gyeongbuk , Republic of Korea .
b Department of Marine Biotechnology , University of Science & Technology , Daejeon , Republic of Korea .
Mitochondrial DNA. 2015;26(6):895-6. doi: 10.3109/19401736.2013.861448. Epub 2014 Jan 10.
The complete mitochondrial genome of the dark-banded rockfish Sebastes inermis was determined using next-generation sequencing, including Sanger sequencing. The full genome was 16,504 bp in length, including 2 rRNA, 22 tRNA and 13 protein-coding genes (PCGs), as well as the control region. The genome was 27.8% A, 26.8% T, 17.1% G and 28.3% C, showing a slight AT bias (54.6%). All PCGs started with an ATG initial codon, except for COX1, which started with a GTG. The stop codon of all PCGs was a TAR, except for Cytb, which contained an incomplete termination codon, T. All tRNAs had the typical clover-leaf structure except tRNA(Ser (AGY)), which lacked the DHU arm. The complete mitogenome of S. inermis will aid the identification and genetic population studies on the genus Sebastes.
利用包括桑格测序在内的下一代测序技术,测定了黑带平鲉(Sebastes inermis)的完整线粒体基因组。该全基因组长度为16,504 bp,包括2个rRNA、22个tRNA和13个蛋白质编码基因(PCGs)以及控制区。基因组中A占27.8%,T占26.8%,G占17.1%,C占28.3%,显示出轻微的AT偏向性(54.6%)。除COX1以GTG起始外,所有PCGs均以ATG起始密码子开始。除细胞色素b(Cytb)含有不完全终止密码子T外,所有PCGs的终止密码子均为TAR。除tRNA(Ser (AGY))缺乏DHU臂外,所有tRNA均具有典型的三叶草结构。黑带平鲉的完整线粒体基因组将有助于平鲉属的鉴定和遗传种群研究。