Jang Yo-Soon, Oh Sung-Yong, Park Kwang-Jae, Kim Ki-Yong, Kim Sung
a East Sea Environment Research Division , Korea Institute of Ocean Science & Technology , Gyeongbuk , Republic of Korea .
b Department of Marine Biotechnology , University of Science & Technology , Daejeon , Republic of Korea .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):65-6. doi: 10.3109/19401736.2013.873902. Epub 2014 Jan 17.
The complete mitogenome of the oblong rockfish Sebastes oblongus was constructed using next-generation sequencing. The full genome was 16,396 bp in length, including two rRNA, 22 tRNA, one control region and 13 protein-coding genes (PCGs). The genome consisted of 28.0% A, 26.4% T, 16.9% G and 28.6% C, showing a slight AT bias (54.4%). All PCGs contained an ATG start codon, excluding COX1 that contained a GTG. All PCGs contained the stop codon TAA, excluding ND3 (TAG stop codon) and Cytb (incomplete termination codon, T). All tRNAs contained the typical clover leaf structure, except for two tRNAs, serine (AGY) and threonine, which lacked the DHU arm. The complete mitogenome of S. oblongus will contribute to genetic analysis of the effective population size for aquaculture.
利用二代测序技术构建了长体平鲉（Sebastes oblongus）的完整线粒体基因组。该全基因组长度为16396 bp，包括两个rRNA、22个tRNA、一个控制区和13个蛋白质编码基因（PCGs）。基因组由28.0%的A、26.4%的T、16.9%的G和28.6%的C组成，显示出轻微的AT偏向性（54.4%）。所有PCGs都含有ATG起始密码子，但COX1含有GTG。所有PCGs都含有终止密码子TAA，但ND3（TAG终止密码子）和Cytb（不完全终止密码子，T）除外。所有tRNAs都含有典型的三叶草叶结构，但丝氨酸（AGY）和苏氨酸的两个tRNAs缺少DHU臂。长体平鲉的完整线粒体基因组将有助于水产养殖有效种群大小的遗传分析。
