Bin Sayeed Muhammad Shahdaat, Shams Tahiatul, Fahim Hossain Sarder, Rahman Md Rezowanur, Mostofa Agm, Fahim Kadir Mohammad, Mahmood Sharif, Asaduzzaman Md
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka-1000, Bangladesh; Department of Pharmacy, University of Asia Pacific, Dhaka-1209, Bangladesh.
Department of Pharmacy, University of Asia Pacific, Dhaka-1209, Bangladesh; Department of Pharmaceutical Technology, University of Dhaka, Dhaka, Bangladesh.
J Ethnopharmacol. 2014 Feb 27;152(1):156-62. doi: 10.1016/j.jep.2013.12.050. Epub 2014 Jan 8.
Previous studies conducted on animals linked consumption of Nigella sativa L. seeds (NS) to decreased anxiety and improved memory. The present study, which was carried out at a boarding school in Bangladesh, was designed to examine probable effect of NS on mood, anxiety and cognition in adolescent human males.
Forty-eight healthy adolescent human males aged between 14 to 17 years were randomly recruited as volunteers and were randomly split into two groups: A (n=24) and B (n=24). The treatment procedure for group A and B were one capsule of 500 mg placebo and 500 mg NS respectively once daily for four weeks. All the volunteers were assessed for cognition with modified California verbal learning test-II (CVLT-II), mood with Bond-Lader scale and anxiety with State-Trait Anxiety Inventory (STAI) at the beginning and after four weeks of either NS or placebo ingestion.
No parameter showed statistically significant variation between A and B in measurements in the beginning, but after 4 weeks of one capsule of NS 500 mg intake, there was statistically significant variation of mood within group B but there was not statistically significant variation between group A and B. No significant variation was found in state anxiety within groups and between group A and B but in case of trait anxiety, significant variation was found within group B but not between group A and B. In case of CVLT II, there was significant variation within B in immediate short-term recall at trial 4 and 5 whereas this difference was found only in case of trial 5 between group A and B. Within group B, short term-free recall, long-term free recall and long-term cued recall had statistical difference whereas between group A and B long-term free recall and long-term cued recall had statistical difference. No parameters had significant variation within group A after placebo intake for 4 weeks.
Over the 4 weeks study period, the use of NS as a nutritional supplement been observed to- stabilize mood, decrease anxiety and modulate cognition positively. However, long term study is suggested before using NS extensively.
先前在动物身上进行的研究表明，食用黑种草籽（NS）与焦虑减轻和记忆力改善有关。本研究在孟加拉国的一所寄宿学校开展，旨在探究NS对青春期男性情绪、焦虑和认知的可能影响。
48名年龄在14至17岁之间的健康青春期男性被随机招募为志愿者，并随机分为两组：A组（n = 24）和B组（n = 24）。A组和B组的治疗程序分别为每天服用一粒500毫克的安慰剂胶囊和500毫克的NS胶囊，持续四周。在摄入NS或安慰剂四周开始时及四周后，所有志愿者均通过改良的加利福尼亚言语学习测试-II（CVLT-II）评估认知，通过邦德-莱德量表评估情绪，通过状态-特质焦虑量表（STAI）评估焦虑。
在开始测量时，A组和B组之间的任何参数均未显示出统计学上的显著差异，但在摄入500毫克NS胶囊四周后，B组内情绪有统计学上的显著差异，但A组和B组之间无统计学上的显著差异。在状态焦虑方面，组内和A组与B组之间均未发现显著差异，但在特质焦虑方面，B组内有显著差异，但A组和B组之间无显著差异。在CVLT II测试中，B组在第4次和第5次试验的即时短期回忆中有显著差异，而A组和B组之间仅在第5次试验中有此差异。在B组内，短期自由回忆、长期自由回忆和长期线索回忆有统计学差异，而A组和B组之间长期自由回忆和长期线索回忆有统计学差异。安慰剂摄入四周后，A组内的任何参数均无显著差异。
在为期四周的研究期间，观察到使用NS作为营养补充剂可稳定情绪、减轻焦虑并对认知产生积极调节作用。然而，在广泛使用NS之前，建议进行长期研究。
