Zhang Yu, Liu Na, Tang Yingxin, Yang Erfang, Dong Shasha, Huang Mengyang, Pan Chao, Zhang Youping, Zhang Ping, Chen Hong, Tang Zhouping
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Mol Neurobiol. 2014 Oct;50(2):647-54. doi: 10.1007/s12035-014-8638-4. Epub 2014 Jan 15.
Neural stem cells (NSCs) can be isolated from nervous tissues or derived from embryonic stem cells. However, their procurement for clinical applications is limited, and there is a need for alternative types of cell that have NSCs properties. In the present study, the differentiation potential of rat adipose-derived stem cells (ADSCs) was evaluated by infecting these cells with a lentiviral vector-encoding green fluorescent protein (GFP). ADSCs transduced with lentivirus were able to generate NSC-like cells, without any effects on their growth, phenotype, and normal differentiation potential. NSC-like cells derived from ADSCs formed neurospheres and expressed high levels of the neural progenitor marker nestin. In the absence of selected growth factors, these neurospheres differentiated into neurons expressing NeuN and MAP2 and GFAP-expressing glia, as determined by immunocytochemistry, Western blotting, and quantitative real-time polymerase chain reaction. These results demonstrate that ADSCs can be induced to generate neurospheres that have NSC-like properties and may thus constitute a potential source of cells in stem cell therapy for neurological disorders.
神经干细胞（NSCs）可从神经组织中分离出来，也可由胚胎干细胞分化而来。然而，用于临床应用时其获取受到限制，因此需要具有神经干细胞特性的其他类型细胞。在本研究中，通过用编码绿色荧光蛋白（GFP）的慢病毒载体感染大鼠脂肪来源干细胞（ADSCs），评估了其分化潜能。用慢病毒转导的ADSCs能够产生神经干细胞样细胞，且对其生长、表型和正常分化潜能无任何影响。源自ADSCs的神经干细胞样细胞形成神经球，并高表达神经祖细胞标志物巢蛋白。在没有特定生长因子的情况下，通过免疫细胞化学、蛋白质免疫印迹和定量实时聚合酶链反应确定，这些神经球分化为表达NeuN和MAP2的神经元以及表达GFAP的神经胶质细胞。这些结果表明，ADSCs可被诱导产生具有神经干细胞样特性的神经球，因此可能成为神经疾病干细胞治疗的潜在细胞来源。
