Ruco L P, Rosati S, Remotti D, Modesti A, Vitolo D, Baroni C D
Histopathology. 1987 Jun;11(6):557-65. doi: 10.1111/j.1365-2559.1987.tb02666.x.
The epithelial, vascular and lymphoid components present in two cases of adenolymphoma (Warthin's tumour) were characterized by immunohistochemistry on frozen sections. Epithelial cells were positive for cytokeratin and keratin and were in close contact with numerous factor VIII related antigen-positive capillary vessels, running parallel to the epithelial basement membrane. The capillary vessels were in communication with high endothelial post-capillary venules which were often lined by HLA-DR-positive endothelial cells. The lymphoid tissue was organized in B-cell and T-cell areas. The B-cell areas consisted of B4+/To15+ B-lymphocytes admixed with DRC-1-positive follicular dendritic reticulum cells. The T-cell areas were mainly populated by T11+/T3+/T4+ lymphocytes admixed with some S-100+/HLA-DR+ interdigitating reticulum cells. Plasma cells and T8+ lymphocytes were more numerous in the proximity of the epithelium. Leu-M3+/PAM-1+ macrophages were scattered singly in the T-cell areas or were arranged in sinus-like structures around the epithelial cysts. The epithelial basement membrane, the vascular membrane and the fibrillar reticular stroma of the lymphoid tissue were immunoreactive for laminin and type IV collagen. The possibility is discussed that the accumulation of lymphoid tissue in Warthin's tumour is modulated by the epithelial cells, perhaps through their ability to organize a peculiar pattern of vascularization.
对两例腺淋巴瘤（沃辛瘤）的冰冻切片进行免疫组织化学分析，以鉴定其中的上皮、血管和淋巴成分。上皮细胞角蛋白和细胞角蛋白呈阳性，与众多因子VIII相关抗原阳性的毛细血管紧密接触，这些毛细血管平行于上皮基底膜排列。毛细血管与高内皮后毛细血管小静脉相通，后者常由HLA - DR阳性的内皮细胞衬里。淋巴组织分为B细胞区和T细胞区。B细胞区由B4 + /To15 + B淋巴细胞与DRC - 1阳性的滤泡树突状网状细胞混合组成。T细胞区主要由T11 + /T3 + /T4 +淋巴细胞与一些S - 100 + /HLA - DR +交错突网状细胞混合组成。上皮附近的浆细胞和T8 +淋巴细胞较多。Leu - M3 + /PAM - 1 +巨噬细胞单个散在于T细胞区，或围绕上皮囊肿排列成窦状结构。淋巴组织的上皮基底膜、血管膜和纤维网状基质对层粘连蛋白和IV型胶原呈免疫反应性。文中讨论了沃辛瘤中淋巴组织的积聚可能受上皮细胞调节的可能性，也许是通过上皮细胞组织特殊血管化模式的能力来实现的。
