Area of Parasitology, University of Zaragoza, Zaragoza, Spain.
Environ Microbiol. 2014 Feb;16(2):339-49. doi: 10.1111/1462-2920.12363. Epub 2014 Jan 15.
The association between free-living amoebae and pathogenic bacteria is an issue that has gained great importance due to the environmental and health consequences that it implies. In this paper, we analyse the techniques to follow an epidemiological study to identify associations between genera, species, genotypes and subgenotypes of amoebae with pathogenic bacteria, analysing their evolution and considering their usefulness. In this sense, we highlight the combination of microscopic and molecular techniques as the most appropriate way to obtain fully reliable results as well as the need to achieve the standardization of these techniques to allow the comparison of both environmental and clinical results.
自由生活阿米巴与致病菌之间的关联是一个非常重要的问题，因为它涉及到环境和健康方面的后果。在本文中，我们分析了进行流行病学研究以确定阿米巴属、种、基因型和亚基因型与致病菌之间关联的技术，分析了它们的进化并考虑了它们的用途。在这方面，我们强调了显微镜技术和分子技术的结合是获得完全可靠结果的最合适方法，以及需要实现这些技术的标准化，以允许比较环境和临床结果。
