Muchesa P, Mwamba O, Barnard T G, Bartie C
Water and Health Research Centre, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa.
Water and Health Research Centre, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa ; National Institute for Occupational Health, P.O. Box 4788, Johannesburg 2000, South Africa.
Biomed Res Int. 2014;2014:575297. doi: 10.1155/2014/575297. Epub 2014 Nov 4.
Free-living amoebae pose a potential health risk in water systems as they may be pathogenic and harbor potential pathogenic bacteria known as amoebae resistant bacteria. Free-living amoebae were observed in 150 (87.2%) of the environmental water samples. In particular, Acanthamoeba sp. was identified in 22 (12.8%) using amoebal enrichment and confirmed by molecular analysis. FLA were isolated in all 8 stages of the wastewater treatment plant using the amoebal enrichment technique. A total of 16 (9.3%) samples were positive for FLA from influent, 20 (11.6%) from bioreactor feed, 16 (9.3%) from anaerobic zone, 16 (9.3%) from anoxic zone, 32 (18.6%) from aerators, 16 (9.3%) from bioreactor effluent, 11 (6.4%) from bioreactor final effluent, and 45 (26.2%) from maturation pond. This study provides baseline information on the occurrence of amoebae in wastewater treatment plant. This has health implications on receiving water bodies as some FLA are pathogenic and are also involved in the transmission and dissemination of pathogenic bacteria.
自由生活阿米巴原虫在水系统中构成潜在的健康风险，因为它们可能具有致病性，并携带被称为耐阿米巴细菌的潜在致病细菌。在150份（87.2%）环境水样中观察到自由生活阿米巴原虫。特别是，通过阿米巴富集法在22份（12.8%）水样中鉴定出棘阿米巴属，并经分子分析确认。使用阿米巴富集技术在污水处理厂的所有8个阶段都分离到了自由生活阿米巴原虫。进水样中共有16份（9.3%）自由生活阿米巴原虫呈阳性，生物反应器进水样中20份（11.6%）呈阳性，厌氧区16份（9.3%）呈阳性，缺氧区16份（9.3%）呈阳性，曝气器中32份（18.6%）呈阳性，生物反应器出水样中16份（9.3%）呈阳性，生物反应器最终出水样中11份（6.4%）呈阳性，熟化池中45份（26.2%）呈阳性。本研究提供了污水处理厂中阿米巴原虫出现情况的基线信息。这对受纳水体具有健康影响，因为一些自由生活阿米巴原虫具有致病性，并且还参与致病细菌的传播和扩散。
