Thakur M S, Ragavan K V
Fermentation Technology and Bioengineering Department, CSIR - Central Food Technological Research Institute, Mysore, 570020 India.
J Food Sci Technol. 2013 Aug;50(4):625-41. doi: 10.1007/s13197-012-0783-z. Epub 2012 Aug 11.
Optical based sensing systems that measure luminescence, fluorescence, reflectance and absorbance, etc., are some of the areas of applications of optical immunosensors. Immunological methods rely on specific binding of an antibody (monoclonal, polyclonal or engineered) to an antigen. Detection of specific microorganisms and microbial toxins requires immobilization of specific antibodies onto a given transducer that can produce signal upon attachment of typical microbe/microbial toxins. Inherent features of immunosensors such as specificity, sensitivity, speed, ease and on-site analysis can be made use for various applications. Safety of food and environment has been a major concern of food technologists and health scientists in recent years. There exists a strong need for rapid and sensitive detection of different components of foods and beverages along with the food borne and water borne pathogens, toxins and pesticide residues with high specificity. Biosensors present attractive, efficient alternative techniques by providing quick and reliable performances. There is a very good potential for application of biosensors for monitoring food quality and safety in food and bioprocessing industries in India.
基于光学的传感系统可测量发光、荧光、反射率和吸光度等,这些是光学免疫传感器的一些应用领域。免疫方法依赖于抗体(单克隆、多克隆或工程化抗体)与抗原的特异性结合。检测特定微生物和微生物毒素需要将特异性抗体固定在给定的换能器上,该换能器在典型微生物/微生物毒素附着时能够产生信号。免疫传感器的固有特性,如特异性、灵敏度、速度、简便性和现场分析能力,可用于各种应用。近年来,食品和环境安全一直是食品技术专家和健康科学家主要关注的问题。迫切需要快速、灵敏地检测食品和饮料的不同成分,以及具有高特异性的食源性病原体、水源性病原体、毒素和农药残留。生物传感器通过提供快速可靠的性能,呈现出有吸引力的高效替代技术。在印度,生物传感器在食品和生物加工行业监测食品质量和安全方面具有很大的应用潜力。