Sundaram Divya, Narayanan Retna Kumari, Vadakkepurayil Kannan
Senior Resident, Department of Pedodontics, Government Dental College , Trivandrum, Kerala, India .
Principal, Government Dental College , Kozhikode, Kerala, India .
J Clin Diagn Res. 2016 Aug;10(8):ZC88-91. doi: 10.7860/JCDR/2016/19268.8311. Epub 2016 Aug 1.
The major determinant of the success of root canal treatment depends on meticulous disinfection of the root canal using intracanal irrigants. The most commonly used root canal irrigant is sodium hypochlorite which has disadvantages of cytotoxicity and unpleasant taste. So there is a need to identify a more biocompatible root canal irrigant.
The aim of this ex-vivo study was to evaluate the efficacy of 40% honey, 100% neem leaf extract and 5.25% sodium hypochlorite as an intracanal irrigant against the isolated microorganisms from infected root canal.
The samples were collected from infected root canals of 60 primary molar teeth indicated for pulpectomy. Alpha hemolytic Streptococci, gram negative bacilli, Candida, Staphylococci, Lactobacilli, Enterococci, Spore bearing gram positive bacilli and Micrococci were the microorganisms isolated from the samples. The zone of inhibition against the microbial growth was measured by agar well diffusion method. Statistical analysis was done by Repeated Analysis of Variance (ANOVA) and Bonferroni method.
Statistical analysis showed that the means of the zones of inhibition measured in this study were 18.56mm, 2.09mm and 1.62mm for sodium hypochlorite, 100% neem leaf extract and 40% honey respectively. The significance was greater between sodium hypochlorite and the other two agents as p-value was <0.001.
The results indicated that 5.25% sodium hypochlorite is more effective as root canal irrigant when compared with 100% neem leaf extract and 40% honey. It was also observed that 100% neem leaf extract has greater antimicrobial effect than 40% honey.
根管治疗成功的主要决定因素取决于使用根管冲洗剂对根管进行细致消毒。最常用的根管冲洗剂是次氯酸钠，它具有细胞毒性和味道不佳的缺点。因此，需要确定一种生物相容性更好的根管冲洗剂。
本体外研究的目的是评估40%蜂蜜、100%印楝叶提取物和5.25%次氯酸钠作为根管冲洗剂对从感染根管分离出的微生物的疗效。
从60颗拟行牙髓摘除术的乳磨牙的感染根管中采集样本。从样本中分离出的微生物有α溶血性链球菌、革兰氏阴性杆菌、念珠菌、葡萄球菌、乳酸杆菌、肠球菌、产芽孢革兰氏阳性杆菌和微球菌。采用琼脂孔扩散法测量对微生物生长的抑制圈。通过重复方差分析（ANOVA）和邦费罗尼方法进行统计分析。
统计分析表明，本研究中测得的次氯酸钠、100%印楝叶提取物和40%蜂蜜抑制圈的平均值分别为18.56毫米、2.09毫米和1.62毫米。次氯酸钠与其他两种试剂之间的差异更显著，p值<0.001。
结果表明，与100%印楝叶提取物和40%蜂蜜相比，5.25%次氯酸钠作为根管冲洗剂更有效。还观察到100%印楝叶提取物比40%蜂蜜具有更强的抗菌作用。
