Department of Restorative Dentistry, University of Ghana Dental School, College of Health Sciences, University of Ghana, Accra, Ghana.
Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
PLoS One. 2024 Jul 8;19(7):e0306693. doi: 10.1371/journal.pone.0306693. eCollection 2024.
Endodontic treatment is one of the main dental treatments to manage inflamed or infected root canal systems of teeth. The success of endodontic treatment principally depends on eradicating microorganisms in the root canal by chemo-mechanical debridement with irrigation solutions like sodium hypochlorite (NaOCl). NaOCl has been used in concentrations ranging from 0.5% to 5.25%. This study determined the antimicrobial effectiveness of selected concentrations (0.5%, 1.0%, 2.6%, and 5.2%) of NaOCl in endodontic treatment.
The study sites were the University of Ghana Dental School (UGDS) and Noguchi Memorial Institute for Medical Research (NMIMR). Sixty infected single-rooted single-canal teeth were used. Before (S1) and after (S2), root canal samples during the endodontic treatment with the selected concentrations of NaOCl were examined via anaerobic and aerobic cultures. The isolates were identified using Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS).
All S1 samples were positive for cultivable bacteria. Fifty-three (53) different microbial species belonging to 20 different microbial genera were isolated. Streptococcus viridans was the most frequently isolated microbe. There were zero isolates in the root canals irrigated with 2.6% and 5.2% NaOCl. Two teeth had isolates in the groups irrigated with the lower concentrations (0.5% and 1.0%) of NaOCl. The persistent bacteria were one species each of Streptococcus mitis and Streptococcus oralis, respectively.
Root canal treatments using chemo-mechanical preparation with the selected concentrations (0.5%, 1.0%, 2.6%, and 5.2%) of NaOCl were effective in significantly reducing the microbial load, and for the 5.2% and 2.6% concentrations, in eliminating all the microorganisms.
根管治疗是治疗牙齿发炎或感染根管系统的主要牙科治疗方法之一。根管治疗的成功主要取决于通过使用次氯酸钠（NaOCl）等冲洗液进行化学机械清创，消除根管内的微生物。NaOCl 的使用浓度范围为 0.5%至 5.25%。本研究旨在确定选定浓度（0.5%、1.0%、2.6%和 5.2%）的 NaOCl 在根管治疗中的抗菌效果。
研究地点为加纳大学牙科学院（UGDS）和古奇纪念医学研究所（NMIMR）。使用了 60 颗感染的单根单根管牙齿。在根管治疗中使用选定浓度的 NaOCl 前后（S1 和 S2），通过厌氧和需氧培养检查根管样本。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定分离株。
所有 S1 样本均培养出可培养细菌。共分离出 53 种不同的微生物，属于 20 种不同的微生物属。变异链球菌是最常分离的微生物。用 2.6%和 5.2%的 NaOCl 冲洗的根管中没有分离株。用较低浓度（0.5%和 1.0%）的 NaOCl 冲洗的两组中有两例分离出细菌。持续存在的细菌分别为缓症链球菌和口腔链球菌。
使用选定浓度（0.5%、1.0%、2.6%和 5.2%）的 NaOCl 进行化学机械制备的根管治疗，可有效显著降低微生物负荷，5.2%和 2.6%的浓度可完全消除所有微生物。
