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观察并描述完整菠菜叶绿体中叶绿素荧光产量的瞬变。

Observation and characterisation of a transient in the yield of chlorophyll fluorescence in intact spinach chloroplasts.

机构信息

Research Institute for Photosynthesis, The University, S10 2TN, Sheffield, UK.

出版信息

Photosynth Res. 1987 Jan;11(2):109-18. doi: 10.1007/BF00018269.

DOI:10.1007/BF00018269
PMID:24435487
Abstract

A transient in chlorophyll fluorescence, which is associated with a transient in 9-aminoacridine fluorescence and a perturbation in the rate of oxygen evolution, has been observed in intact spinach chloroplasts. The results indicate that changes in the redox state of Q are, at least partially, responsible for the transient in chlorophyll fluorescence. The size of the transient is highly dependent upon the concentration of inorganic phosphate and upon the pH of the medium. The properties of the transient are consistent with the suggestion that it reflects changes in the levels of stromal intermediates during induction.

摘要

在完整的菠菜叶绿体中观察到叶绿素荧光的瞬时变化,该变化与 9-氨基吖啶荧光的瞬时变化和氧释放速率的扰动有关。结果表明,Q 的氧化还原状态的变化至少部分地导致了叶绿素荧光的瞬时变化。瞬变的大小高度依赖于无机磷酸盐的浓度和介质的 pH 值。瞬变的性质与以下建议一致,即它反映了诱导过程中基质中间产物水平的变化。

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本文引用的文献

1
Sudden changes in the rate of photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts: the role of orthophosphate.完整分离叶绿体中光合放氧和叶绿素荧光速率的突然变化:正磷酸盐的作用。
Photosynth Res. 1987 Jan;11(2):119-30. doi: 10.1007/BF00018270.
2
Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation.菠菜叶片次生荧光动力学与光合作用碳同化起始的关系。
Planta. 1981 Nov;153(3):273-8. doi: 10.1007/BF00383899.
3
Simultaneous measurement of oscillations in oxygen evolution and chlorophyll a fluorescence in leaf pieces.
用再照光诱导完整的分离豌豆叶绿体中的叶绿素荧光振荡。
Planta. 1991 Oct;185(3):397-400. doi: 10.1007/BF00201063.
同时测量叶片中氧释放和叶绿素 a 荧光的振荡。
Plant Physiol. 1983 Nov;73(3):542-9. doi: 10.1104/pp.73.3.542.
4
Adenine nucleotide status, phosphoglycerate reduction and photosynthetic phosphorylation in a reconstituted chloroplast system.重组叶绿体系统中的腺嘌呤核苷酸状态、磷酸甘油酸还原与光合磷酸化
Biochem J. 1983 Jan 15;210(1):273-6. doi: 10.1042/bj2100273.
5
The reduction of 3-phosphoglycerate by reconstituted chloroplasts and by chloroplast extracts.通过重组叶绿体和叶绿体提取物对3-磷酸甘油酸的还原作用。
Biochim Biophys Acta. 1974 Dec 19;368(3):269-78. doi: 10.1016/0005-2728(74)90174-1.
6
The relationship between light scattering and chlorophyll a fluorescence during oscillations in photosynthetic carbon assimilation.光合碳同化振荡过程中光散射与叶绿素a荧光之间的关系。
Arch Biochem Biophys. 1985 Mar;237(2):513-9. doi: 10.1016/0003-9861(85)90304-2.
7
The control of 3-phosphoglycerate reduction in isolated chloroplasts by the concentrations of ATP, ADP and 3-phosphoglycerate.通过ATP、ADP和3-磷酸甘油酸的浓度对分离叶绿体中3-磷酸甘油酸还原的控制。
Biochim Biophys Acta. 1979 Mar 15;545(3):528-36. doi: 10.1016/0005-2728(79)90161-0.