Spicer S S, Erlandsen S L, Wilson A C, Hammer M F, Hennigar R A, Schulte B A
Department of Pathology, Medical University of South Carolina, Charleston 29425.
J Histochem Cytochem. 1987 Nov;35(11):1231-44. doi: 10.1177/35.11.2443557.
A wide range of tissues from three interfertile species of mice and an interspecific hybrid was examined with lectins conjugated to peroxidase to localize specifically glycoconjugates containing terminal alpha-N-acetylgalactosamine, alpha-galactose, and alpha-fucose, and the terminal disaccharide galactose-(beta 1----3)-N-acetylgalactosamine. This battery of lectins disclosed marked heterogeneity of glycoconjugates in different histological sites in a given animal and even between cells in a presumably homogeneous cell population within an organ. No variation with any lectin was observed between individuals of two closely related inbred strains of Mus domesticus at any specific histological or cytological site. In contrast, littermates of an outbred strain of Mus castaneus differed in binding of certain lectins at various sites, attesting to a genetic basis for individual variation. Hybrids between castaneus and domesticus mice also showed individual variation. Moreover, extensive differences between the mouse species were demonstrable with every lectin in glycoconjugates of stored secretions, Golgi cisternae, and apical or basolateral plasmalemma in many cell types. Totaling the differences in tabulated staining intensities for each possible species pair gave a measure of the overall extent of difference at 53 histological sites. According to this measure, the three species are about equally divergent from one another. Some differences between species appeared to depend on histological rather than histochemical variation, as, for example, a greater abundance of granular duct cells in the sublingual and submandibular glands in Mus hortulanus. Other differences were apparently derived from pathological change, as exemplified by casts and lymphoid infiltrates in kidney and structurally atypical submandibular gland lobules in Mus castaneus, and possibly by infiltrating cells in intestinal lamina propria and epithelium in Mus castaneus and hortulanus.
利用与过氧化物酶结合的凝集素对三种可杂交的小鼠物种以及一个种间杂交体的多种组织进行了检查，以特异性定位含有末端α-N-乙酰半乳糖胺、α-半乳糖和α-岩藻糖的糖缀合物，以及末端二糖半乳糖-(β1→3)-N-乙酰半乳糖胺。这一系列凝集素揭示了给定动物不同组织学部位的糖缀合物存在显著异质性，甚至在一个器官内假定均匀的细胞群体中的细胞之间也是如此。在家养小鼠的两个密切相关的近交系个体之间，在任何特定的组织学或细胞学部位，未观察到与任何凝集素的差异。相比之下，野生栗色小鼠杂种的同窝仔在不同部位对某些凝集素的结合存在差异，证明个体变异具有遗传基础。栗色小鼠和家养小鼠之间的杂种也表现出个体变异。此外，在许多细胞类型的储存分泌物、高尔基池以及顶端或基底外侧质膜的糖缀合物中，每种凝集素都显示出小鼠物种之间存在广泛差异。将每个可能的物种对的染色强度差异列表汇总，得出了53个组织学部位的总体差异程度。根据这一衡量标准，这三个物种彼此之间的差异大致相同。物种之间的一些差异似乎取决于组织学而非组织化学变化，例如，园小鼠舌下腺和下颌下腺中的颗粒导管细胞数量更多。其他差异显然源于病理变化，例如栗色小鼠肾脏中的管型和淋巴浸润以及结构非典型的下颌下腺小叶，可能还源于栗色小鼠和园小鼠肠道固有层和上皮中的浸润细胞。
