Geng Peiliang, Yao Jie, Zhu Yunfeng
The Key Lab, Cancer Center, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.
Mol Biol Rep. 2014;41(4):2299-306. doi: 10.1007/s11033-014-3083-z. Epub 2014 Jan 17.
The Ser326Cys polymorphism in the human 8-oxogunaine glycosylase (hOGG1) gene with lung cancer susceptibility had been investigated by the approaches of PCR-RFLP, PCR-SSCP and ASA. Due to limited specimen and different approaches the conclusion was drawn toughly. To evaluate this correlation comprehensively, a meta-analysis was performed based on 30 case-control studies, including 10,327 cases and 12,148 controls. The random-effects model was used to estimate the odds ratios and 95 % confidence interval for various contrasts of this polymorphism. The combined results suggested that the hOGG1 Ser326Cys polymorphism was not associated with lung cancer susceptibility in different genetic models. Similarly, in the stratified analyses by ethnicity and source of control, no risk was observed between all the genetic models and lung cancer risk. Our meta-analysis revealed that there was little correlation between the hOGG1 Ser326Cys polymorphism and the risk of lung cancer.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）、聚合酶链反应-单链构象多态性（PCR-SSCP）和等位基因特异性扩增（ASA）等方法，对人类8-氧代鸟嘌呤糖基化酶（hOGG1）基因中的Ser326Cys多态性与肺癌易感性进行了研究。由于样本有限且方法不同，得出的结论较为勉强。为全面评估这种相关性，基于30项病例对照研究进行了荟萃分析，其中包括10327例病例和12148例对照。采用随机效应模型估计该多态性各种对比的比值比及95%置信区间。综合结果表明，在不同遗传模型中，hOGG1 Ser326Cys多态性与肺癌易感性无关。同样，在按种族和对照来源进行的分层分析中，所有遗传模型与肺癌风险之间均未观察到风险。我们的荟萃分析表明，hOGG1 Ser326Cys多态性与肺癌风险之间几乎没有相关性。
