体内光遗传学鉴定和操纵 GABA 能中间神经元亚型。
In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes.
机构信息
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States.
NYU Neuroscience Institute, School of Medicine and Center for Neural Science, New York University, New York, NY 10016, United States; Department of Psychiatry, New York University Medical Center, New York, NY 10016, United States.
出版信息
Curr Opin Neurobiol. 2014 Jun;26:88-95. doi: 10.1016/j.conb.2013.12.013. Epub 2014 Jan 14.
Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.
在行为动物中识别和操作不同的 GABA 能中间神经元类型对于了解它们在回路动力学和行为中的作用非常重要。光遗传学和大规模神经元记录的结合允许在完整动物中识别和干扰特定的中间神经元群体，以进行回路分析。这种方法的一个关键方面是将电生理记录与空间和时间精确的光传递相结合。以预定的时间配置或闭环方式对神经元激活剂和沉默剂进行焦点多点照明，为解决许多新问题打开了大门。最近的进展证明了这种新技术对于中间神经元研究的实用性和强大功能。
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