Joukar Siyavash, Najafipour Hamid, Dabiri Shahriar, Sheibani Mohammad, Sharokhi Nader
Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran,
Cardiovasc Toxicol. 2014 Sep;14(3):214-21. doi: 10.1007/s12012-014-9245-3.
This study assessed the effects of mumie (shilajit) pre-treatment, a traditional drug which is well known in the ancient medicine of both east and west, on cardiac performance of rats subjected to myocardial injury. Animals were divided into control, M250, and M500 (received mumie at dosages of 250 and 500 mg/kg/day, orally for 7 days, respectively) main groups each consisting of two subgroups-with and without heart injury. On the 6th and 7th days, isoproterenol (ISO) (85 mg/kg i.p.) was injected (s.c.) to half of the animal subgroups to induce myocardial damage. On the 8th day, after hemodynamic parameter recordings, hearts were removed for further evaluation. Mumie pre-treatment had no significant effects on hemodynamic and cardiac indices of normal animals. When the cardiac injury was induced, mumie maintained the ±dp/dt maximum, attenuated the serum cardiac troponin I, and reduced the severity of cardiac lesions. Despite the mild positive effects of mumie on total antioxidant capacity and lipid proxidation index, no significant difference was observed among animal groups. The findings suggest the prominent cardioprotective effect of mumie against destructive effects of ISO. It seems that other mechanisms than reinforcements of antioxidant system are involved in this beneficial effect.
本研究评估了密里（希拉季特）预处理对心肌损伤大鼠心脏功能的影响。密里是一种传统药物，在东西方古代医学中都广为人知。动物被分为对照组、M250组和M500组（分别以250和500毫克/千克/天的剂量口服密里7天），每个主要组又分为有心脏损伤和无心脏损伤两个亚组。在第6天和第7天，对一半动物亚组皮下注射异丙肾上腺素（ISO）（85毫克/千克腹腔注射）以诱导心肌损伤。在第8天，记录血流动力学参数后，取出心脏进行进一步评估。密里预处理对正常动物的血流动力学和心脏指标无显著影响。当诱导心脏损伤时，密里维持最大±dp/dt，降低血清心肌肌钙蛋白I水平，并减轻心脏病变的严重程度。尽管密里对总抗氧化能力和脂质过氧化指数有轻微的积极影响，但各动物组之间未观察到显著差异。研究结果表明密里对ISO的破坏作用具有显著的心脏保护作用。似乎除了增强抗氧化系统外，其他机制也参与了这种有益作用。
