Univ Bordeaux, ISVV, EA 4577 Œnologie, Villenave d'Ornon F-33140, France.
School of Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
Molecules. 2014 Jan 2;19(1):482-506. doi: 10.3390/molecules19010482.
Grenache, Syrah, Carignan Noir, Mourvèdre, Counoise and Alicante Bouchet grape seeds and skins, harvested in 2009 and 2010 in the Rhône valley area of France, and their respective pomaces remaining after vinification, were analyzed for their phenolic composition and antioxidant activity. The polyphenol content was quantified by HPLC and the Folin-Ciocalteu assay. The antioxidant potential was measured with four different assays: ORAC, FRAP, ABTS and DPPH. Seeds contained higher amounts of total polyphenols, up to 44.5 mg of gallic acid equivalent [GAE]/g dry weight in Alicante pomace, than skin extracts. The maximum total phenolic in skins was 31.6 mg GAE/g dry weight detected in 2010 Alicante pomace. Seeds also had the highest antioxidant capacity. HPLC analysis revealed that, despite the vinification process, pomaces still contained an appreciable amount of proanthocyanidins as well as several anthocyanin glycosides. Alicante and Syrah proved to be the varieties of most interest in terms of their potential development for nutraceutical purposes.
歌海娜、西拉、黑佳丽酿、慕合怀特、神索和白玉霓的葡萄种子和果皮，于 2009 年和 2010 年在法国罗纳河谷地区收获，以及各自的酿酒剩余果渣，对其酚类成分和抗氧化活性进行了分析。多酚含量通过 HPLC 和福林-考尔法特（Folin-Ciocalteu）测定进行定量。抗氧化能力通过四种不同的方法进行测量：ORAC、FRAP、ABTS 和 DPPH。种子中的总多酚含量高于果皮提取物，高达 44.5 毫克没食子酸当量（GAE）/克干重，在 Alicante 果渣中达到 44.5 毫克。在 2010 年 Alicante 果渣中，果皮中总酚含量最高，为 31.6 毫克 GAE/g 干重。种子的抗氧化能力也最高。HPLC 分析表明，尽管经过酿酒过程，果渣仍含有相当数量的原花青素以及几种花色苷糖苷。就其作为营养保健品开发的潜力而言，Alicante 和西拉被证明是最有价值的品种。
