Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.
Environ Sci Pollut Res Int. 2014 May;21(9):6016-24. doi: 10.1007/s11356-014-2543-2. Epub 2014 Jan 25.
Formaldehyde is classified as a human carcinogen that may cause nasopharyngeal cancer and probably leukemia. The effects of environmental and nutritional factors on fungal growth and the biodegradation of formaldehyde were investigated. Fungal strains SGFA1 and SGFA3 isolated from untreated sewage sediment samples collected from heavily formaldehyde-contaminated areas were identified using morphological characteristics and molecular techniques and named as Aspergillus nomius SGFA1 and Penicillium chrysogenum SGFA3. Results indicate that SGFA1 and SGFA3 completely consumed 3,000 and 900 mg l(-1) of formaldehyde, respectively, within 7 days under optimized conditions. Quantitative real-time PCR analyses and enzyme activity analyses demonstrated that glutathione-dependent formaldehyde dehydrogenase (GDFADH) and formate dehydrogenase (FDH) pathway may play a functional role in enhancing formaldehyde-degrading capability in SGFA1. Both fungi have potential use for remediation of formaldehyde pollution.
甲醛被归类为人类致癌物，可能导致鼻咽癌和白血病。本研究调查了环境和营养因素对真菌生长和甲醛生物降解的影响。从甲醛污染严重地区采集的未经处理的污水沉积物样本中分离出的真菌菌株 SGFA1 和 SGFA3，通过形态特征和分子技术进行鉴定，并分别命名为曲霉 nomius SGFA1 和青霉 chrysogenum SGFA3。结果表明，在优化条件下，SGFA1 和 SGFA3 分别在 7 天内完全消耗了 3000 和 900mg/L 的甲醛。定量实时 PCR 分析和酶活性分析表明，谷胱甘肽依赖型甲醛脱氢酶（GDFADH）和甲酸盐脱氢酶（FDH）途径可能在增强 SGFA1 甲醛降解能力方面发挥功能作用。这两种真菌都具有修复甲醛污染的潜力。
