Wei Lihui, Shao Ying, Wan Jingwang, Feng Hui, Zhu Hua, Huang Huiwen, Zhou Yijun
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, People's Republic of China.
PLoS One. 2014 Jan 21;9(1):e85988. doi: 10.1371/journal.pone.0085988. eCollection 2014.
The rhizobacterial strain Jdm2 was isolated from the rhizosphere of the traditional Chinese medicinal herb Trichosanthes kirilowii in Jiangsu province, China, and was identified as Bacillus subtilis. Exposure of cell-free filtrate of the strain to the root-knot nematode Meloidogyne incognita under in vitro conditions caused substantial mortality of the second stage juvenile (J2) and significantly reduced egg hatchability. A greenhouse trial demonstrated that 56 days after treatment with Jdm2, the number of galls associated with M. incognita infection in the tomato (Solanum lycopersicum) roots was significantly reduced compared to controls, and the disease severity of infected plants was lower in treated plants (36%) compared to water control (75%). Consistently, in the field trial, the biocontrol efficacy of Jdm2 reached 69%, 51% and 48% after 30, 60 and 90 days post-transplantation, respectively. As indicated by PCR-DGGE analysis, inoculation with Jdm2 strain had an effect on the bacterial community of the tomato rhizosphere at the first stage, but was not able to imperil the bacterial community stability for long time. The novel bacterial strain Jdm2 enhances plant growth and inhibits nematode activity, and has the potential to be a safe and effective microbial pesticide.
根际细菌菌株Jdm2是从中国江苏省传统中药材栝楼的根际分离得到的，经鉴定为枯草芽孢杆菌。在体外条件下，将该菌株的无细胞滤液暴露于南方根结线虫，导致第二代幼虫（J2）大量死亡，并显著降低了卵的孵化率。温室试验表明，用Jdm2处理56天后，与未处理的对照相比，番茄（Solanum lycopersicum）根中与南方根结线虫感染相关的虫瘿数量显著减少，且处理植株中受感染植株的病情严重程度低于用水处理的对照植株（36%对75%）。同样，在田间试验中，Jdm2在移植后30、60和90天的生物防治效果分别达到69%、51%和48%。PCR-DGGE分析表明，接种Jdm2菌株在第一阶段对番茄根际细菌群落有影响，但不能长期危及细菌群落的稳定性。新型细菌菌株Jdm2促进植物生长并抑制线虫活性，有潜力成为一种安全有效的微生物农药。
