植入工程软骨的小鼠颅骨缺损的显微CT切片与组织学切片之间的相关性
CORRELATION BETWEEN MICRO-CT SECTIONS AND HISTOLOGICAL SECTIONS OF MOUSE SKULL DEFECTS IMPLANTED WITH ENGINEERED CARTILAGE.
作者信息
Duke P J, Doan L, Luong H, Kelley C, Leboeuf W, Diep Q, Johnson E, Cody D D
机构信息
Department of Orthodontics, Dental Branch, UTHSC, Houston, TX.
Our Lady of the Lake College, Baton Rouge, LA.
出版信息
Gravit Space Biol Bull. 2009 Sep;22(2):45-50.
One advantage of using cartilage to replace/repair bone is that the implant disappears as bone is formed by endochondral ossification. Previously, we showed that cartilage spheroids, grown in a rotating bioreactor (Synthecon, Inc.) and implanted into a 2 mm skull defect, contributed to healing of the defect. Skulls with or without implants were subjected to microCT scans. Mineralized regions from microCT sections correlated with regions of bone in histological sections of the defect region of demineralized skulls. Recently, sections from microCT scans of live mice were compared to histological sections from the same mice. The area of the defect staining for bone in histological sections of demineralized skulls was the same region shown as mineralized in microCT sections. Defects without implants were not healed. This study demonstrates that microCT scans are an important corollary to histological studies evaluating the use of implants in healing of bony defects.
使用软骨替代/修复骨骼的一个优点是，随着软骨内成骨形成骨骼，植入物会消失。此前，我们发现，在旋转生物反应器（Synthecon公司）中培养并植入2毫米颅骨缺损处的软骨球体有助于缺损的愈合。对有或没有植入物的颅骨进行了微型计算机断层扫描（microCT）。microCT切片中的矿化区域与脱钙颅骨缺损区域组织学切片中的骨区域相关。最近，将活体小鼠microCT扫描的切片与同一只小鼠的组织学切片进行了比较。脱钙颅骨组织学切片中骨染色的缺损区域与microCT切片中显示为矿化的区域相同。没有植入物的缺损没有愈合。这项研究表明，microCT扫描是评估植入物在骨缺损愈合中应用的组织学研究的重要补充。
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